Babitha Sumathy, Soccol Carlos Ricardo, Pandey Ashok
Biotechnology Division, National Institute for Interdisciplinary Science and Technology, CSIR, Trivandrum, India.
J Basic Microbiol. 2007 Apr;47(2):118-26. doi: 10.1002/jobm.200610261.
The aim of the current work was to study the influence of stress on pigment production in Monascus purpureus under solid-state fermentation. Thermal stress was induced by incubating the culture at various high temperatures. For giving osmotic stress, different concentration of NaCl and glycerol were added to the solid substrate prior to autoclaving. Morphological studies were done by light microscopy and scanning electron microscopy. When the cells were exposed to high temperature, high glycerol and salt concentration, significant changes in pigment production and growth were observed. High temperature (>45 degrees C) induced the production of more yellow pigments. High concentration of NaCl induced conidiation and caused a decrease in fungal biomass (up to 50%) but red pigment production increased from 11.86 OD/gds to 20.14 OD/gds. When subjected to glycerol stress, a significant increase in aerial mycelia was observed when compared with the control conditions. The results attain significance for exploiting the fungal culture of Monascus purpureus LPB 97 for producing colors of choice--red, or yellow, or increasing the yield of red pigments considerably. Thus, these results could well impact the commercial aspect of Monascus pigments for industrial application.
当前工作的目的是研究固态发酵条件下压力对紫红红曲霉菌色素产生的影响。通过在不同高温下培养培养物来诱导热应激。为了施加渗透应激，在高压灭菌前向固体底物中添加不同浓度的氯化钠和甘油。通过光学显微镜和扫描电子显微镜进行形态学研究。当细胞暴露于高温、高甘油和盐浓度时，观察到色素产生和生长的显著变化。高温（>45℃）诱导产生更多的黄色色素。高浓度的氯化钠诱导分生孢子形成，并导致真菌生物量减少（高达50%），但红色色素产量从11.86 OD/gds增加到20.14 OD/gds。当受到甘油应激时，与对照条件相比，气生菌丝体显著增加。这些结果对于利用紫红红曲霉菌LPB 97的真菌培养物生产所需颜色（红色或黄色）或大幅提高红色色素产量具有重要意义。因此，这些结果可能会对红曲霉菌色素在工业应用中的商业方面产生重大影响。
