Ndi Chi P, Semple Susan J, Griesser Hans J, Barton Mary D
Sansom Institute, School of Pharmacy and Medical Sciences, University of South Australia, Frome Road, Adelaide, SA, Australia.
J Basic Microbiol. 2007 Apr;47(2):158-64. doi: 10.1002/jobm.200610262.
Plant species of the genus Eremophila (Myoporaceae) are native to Australia and are known to produce a diverse range of unusual secondary compounds. The purpose of this research was to examine the antimicrobial activity of 72 Eremophila species most of which had not been the subject of any previous pharmacological testing. Organic extracts of Eremophila species were screened for antimicrobial activity against Gram-positive and Gram-negative bacteria and yeasts of medical importance. Extracts of a number of Eremophila species showed selective activity against Gram-positive bacteria with MICs for the most active species in the range of 16 to 62 microg/ml for Streptococcus species, and 62 to 250 microg/ml for standard strains of Staphylococcus aureus. Extracts with the greatest activity against standard strains were tested against 68 clinical isolates of multi-resistant methicillin-resistant S. aureus (mMRSA). The majority of the clinical isolates were susceptible to concentrations below 62.5 microg/ml for the extracts of E. drummondii, E. linearis, E. serrulata, E. acrida, E. neglecta, E. virens and a new undescribed species affiliated with E. prolata. The extract of E. virens inhibited growth of all 68 clinical mMRSA isolates at the minimum tested concentration of 31 microg/ml. This study has shown for the first time that a number of different Eremophila species manifest selective antibacterial activity against Gram-positive organisms which are important causes of human disease. It shows that there are several Eremophila species possessing interesting antibacterial activity besides those that have published traditional use. These may yield novel antibacterial compounds with potential to be used in biomedical applications.
埃雷莫菲拉属（苦槛蓝科）的植物物种原产于澳大利亚，已知能产生多种不同寻常的次生化合物。本研究的目的是检测72种埃雷莫菲拉属植物的抗菌活性，其中大多数此前未曾进行过任何药理测试。对埃雷莫菲拉属植物的有机提取物进行了筛选，以检测其对具有医学重要性的革兰氏阳性菌、革兰氏阴性菌和酵母菌的抗菌活性。许多埃雷莫菲拉属植物的提取物对革兰氏阳性菌表现出选择性活性，对活性最强的物种而言，对链球菌属的最低抑菌浓度在16至62微克/毫升范围内，对金黄色葡萄球菌标准菌株的最低抑菌浓度在62至250微克/毫升范围内。对标准菌株活性最强的提取物针对68株多重耐药耐甲氧西林金黄色葡萄球菌（mMRSA）临床分离株进行了测试。对于德拉蒙德埃雷莫菲拉、线状埃雷莫菲拉、锯齿状埃雷莫菲拉、尖锐埃雷莫菲拉、被忽视埃雷莫菲拉、绿色埃雷莫菲拉以及与长叶埃雷莫菲拉相关的一个未描述新物种的提取物，大多数临床分离株对浓度低于62.5微克/毫升的提取物敏感。绿色埃雷莫菲拉的提取物在最低测试浓度31微克/毫升时抑制了所有68株临床mMRSA分离株的生长。本研究首次表明，多种不同的埃雷莫菲拉属植物对作为人类疾病重要病因的革兰氏阳性菌表现出选择性抗菌活性。研究表明，除了那些已公布有传统用途的埃雷莫菲拉属植物外，还有几种具有有趣的抗菌活性。这些植物可能会产生具有用于生物医学应用潜力的新型抗菌化合物。
