Zampini I C, Cuello S, Alberto M R, Ordoñez R M, D' Almeida R, Solorzano E, Isla M I
INQUINOA (CONICET), Ayacucho 471, 4000 San Miguel de Tucumán, Argentina.
J Ethnopharmacol. 2009 Jul 30;124(3):499-505. doi: 10.1016/j.jep.2009.05.011. Epub 2009 May 23.
The plant species reported here are traditionally used in the "Puna" or "Altiplano" of Argentina for ailments related to bacterial infections. The aim of this study was to evaluate their antimicrobial properties against a panel of sensitive and multi-resistant gram-positive and gram-negative bacteria.
The antimicrobial activity of tinctures and aqueous extracts (Baccharis boliviensis, Chiliotrichiopsis keidelii, Chuquiraga atacamensis, Fabiana bryoides, Fabiana densa, Fabiana punensis, Frankenia triandra, Parastrephia lucida, Parastrephia lepidophylla, Parastrephia phyliciformis, Tetraglochin cristatum) was determined using the agar macrodilution and broth microdilution methods recommended by the Clinical and Laboratory Standards Institute (CLSI, formerly NCCLS). The antibiotic resistant clinical strains were isolated from nosocomial infection in human lesions of skin and soft parts.
The ethanolic extracts of 11 plant species inhibited the growth of one or more of the following strains: Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Enterobacter cloacae, Morganella morganii, Pseudomonas aeruginosa. Ethanol extracts (tinctures) of aerial parts of Baccharis, Fabiana and Parastrephia showed the highest levels of antibacterial activity on methicillin, oxacillin and gentamicin resistant Staphylococcus with MIC values from 20 to 150 microg/ml. Baccharis boliviensis and Fabiana bryoides were more active than the other plant species on Enterococcus faecalis with different phenotype. The most interesting activity on multi-resistant gram-negative strains was obtained from Chuquiraga atacamensis. Parastrephia species showed activity against Enterobacter cloacae, Pseudomonas aeruginosa and Proteus mirabilis. The ethanolic extracts exhibited stronger activity and broader spectrum of action than aqueous extracts. The extracts were bactericidal in most cases.
The presence of antibacterial activity in Puna plant extracts against multi-resistant bacteria give support to their traditional use for treating conditions associated with microorganisms in humans and animals and consequently seems promising for the treatment of multi-resistant bacteria.
本文报道的植物物种在阿根廷的“普纳”或“阿尔蒂普拉诺”地区传统上用于治疗与细菌感染相关的疾病。本研究的目的是评估它们对一组敏感和多重耐药的革兰氏阳性及革兰氏阴性细菌的抗菌特性。
采用临床和实验室标准协会（CLSI，原NCCLS）推荐的琼脂稀释法和肉汤微量稀释法，测定酊剂和水提取物（玻利维亚巴卡里斯、凯氏智利蓟、阿塔卡马楚基拉加、苔藓法比亚纳、密集法比亚纳、普纳法比亚纳、三蕊瓣鳞花、光亮副蜡菊、鳞叶副蜡菊、叶状副蜡菊、 cristatum四棱草）的抗菌活性。抗生素耐药临床菌株从人类皮肤和软组织损伤的医院感染中分离得到。
11种植物的乙醇提取物抑制了以下一种或多种菌株的生长：金黄色葡萄球菌、粪肠球菌、大肠杆菌、肺炎克雷伯菌、奇异变形杆菌、阴沟肠杆菌、摩根摩根菌、铜绿假单胞菌。巴卡里斯、法比亚纳和副蜡菊地上部分的乙醇提取物（酊剂）对耐甲氧西林、苯唑西林和庆大霉素的金黄色葡萄球菌表现出最高水平的抗菌活性，最低抑菌浓度值为20至150微克/毫升。玻利维亚巴卡里斯和苔藓法比亚纳对不同表型的粪肠球菌比其他植物物种更具活性。对多重耐药革兰氏阴性菌株最有趣的活性来自阿塔卡马楚基拉加。副蜡菊属植物对阴沟肠杆菌、铜绿假单胞菌和奇异变形杆菌表现出活性。乙醇提取物比水提取物表现出更强的活性和更广泛的作用谱。在大多数情况下，提取物具有杀菌作用。
普纳植物提取物对多重耐药细菌具有抗菌活性，这支持了它们在治疗人类和动物与微生物相关疾病方面的传统用途，因此在治疗多重耐药细菌方面似乎很有前景。
