Lin Haichao, Wang Xin, Liu Guifen, Fu Jinlian, Wang Aiguo
College of Animal Science and Technology, China Agricultural University, Beijing, People's Republic of China.
Mol Reprod Dev. 2007 Nov;74(11):1379-85. doi: 10.1002/mrd.20732.
Integrin are adhesion molecules involved in uterine-conceptus interactions during the perimplantation period. In this study, the expression of alphaV and beta3 integrin subunits in endometrium during implantation in pigs was investigated. The immunohistochemical location was performed on paraformaldehyde-fixed, paraffin-embedded tissue sections, and the mRNA expression of alphaV was detected in endometrium. In addition, serum levels of estradiol, progesterone, follicle-stimulating hormone, and luteinizing hormone were measured on Days 0, 12, 18, and 25 of pregnancy. The results indicate that endometrium expressed integrin alphaV and beta3 in all stages examined. The most intensive staining for integrin alphaV and beta3 was observed in endometrial stroma in porcine pregnancy on Day 18. The mRNA of alphaV integrin strongly expressed on Day 18, and moderately expressed on Days 12 and 25. The correlation between serum hormone level and the mRNA expression of alphaV integrin was not significant. The expression patterns of integrin alphaV and beta3 during implantation provide insights into the important physiological function of alphaVbeta3 integrin in pig, and the strong expression of integrin alphaV and beta3 in mid-implantation may indicate its crucial role in successful implantation and embryo survival.
整合素是在着床前期参与子宫与孕体相互作用的黏附分子。在本研究中，对猪着床期间子宫内膜中αV和β3整合素亚基的表达进行了研究。对用多聚甲醛固定、石蜡包埋的组织切片进行免疫组织化学定位，并检测子宫内膜中αV的mRNA表达。此外，在妊娠第0、12、18和25天测量血清雌二醇、孕酮、促卵泡激素和促黄体生成素水平。结果表明，在所检测的所有阶段，子宫内膜均表达整合素αV和β3。在妊娠第18天的猪子宫内膜基质中观察到整合素αV和β3的染色最为强烈。αV整合素的mRNA在第18天强烈表达，在第12天和第25天中度表达。血清激素水平与αV整合素的mRNA表达之间的相关性不显著。着床期间整合素αV和β3的表达模式为了解αVβ3整合素在猪中的重要生理功能提供了线索，并且整合素αV和β3在着床中期的强烈表达可能表明其在成功着床和胚胎存活中的关键作用。
