Reproductive Medical Center, Department of Obstetrics and Gynaecology, Central South University, Changsha 410008, PR China.
Eur J Obstet Gynecol Reprod Biol. 2013 Sep;170(1):171-6. doi: 10.1016/j.ejogrb.2013.06.019. Epub 2013 Jul 17.
To explore the roles of osteopontin and β3 integrin in successful implantation.
In this study, an early pregnant mouse model was established by peritoneal injection of pregnant mare serum gonadotropin and human chorionic gonadotropin (PMSG+hCG). The expression of osteopontin (OPN) and β3 integrin on the endometrium was measured by immunohistochemistry, RT-PCR, and western blot. The function of OPN and β3 integrin in implantation was investigated by intrauterine injection of OPN and β3 integrin antibody.
We found that PMSG+hCG injection significantly increased the number of blastocysts during implantation as well as the concentration of estradiol and progesterone in serum and endometrium tissues. OPN and β3 integrin were co-expressed in luminal epithelium and their levels dynamically changed from day 4 to day 8 of pregnancy with peak expression on day 5. The percentages of OPN and β3 integrin positive cells in the luminal epithelium were significantly higher in PMSG+hCG-stimulated mice on day 5 than in control mice. Functional blockade of OPN and β3 integrin significantly inhibited implantation.
This study suggests that co-expression of OPN and β3 integrin is a biological marker for good endometrial receptivity and that both proteins play a crucial role in blastocyst implantation.
探讨骨桥蛋白和β3 整合素在成功着床中的作用。
本研究通过腹腔注射孕马血清促性腺激素和人绒毛膜促性腺激素（PMSG+hCG）建立早期妊娠小鼠模型。通过免疫组织化学、RT-PCR 和 Western blot 检测子宫内膜骨桥蛋白（OPN）和β3 整合素的表达。通过宫内注射 OPN 和β3 整合素抗体研究 OPN 和β3 整合素在着床中的作用。
我们发现，PMSG+hCG 注射显著增加了着床期间的胚泡数量以及血清和子宫内膜组织中雌二醇和孕酮的浓度。OPN 和β3 整合素在腔上皮中共同表达，其水平从妊娠第 4 天到第 8 天动态变化，在第 5 天达到峰值。在 PMSG+hCG 刺激的小鼠中，第 5 天腔上皮中 OPN 和β3 整合素阳性细胞的百分比明显高于对照组。OPN 和β3 整合素的功能阻断显著抑制了着床。
本研究表明，OPN 和β3 整合素的共表达是良好子宫内膜容受性的生物学标志物，这两种蛋白在胚泡着床中都起着至关重要的作用。
