Zabrouskov Vlad, Whitelegge Julian P
Thermo Fisher, San Jose, California, California 95134, USA.
J Proteome Res. 2007 Jun;6(6):2205-10. doi: 10.1021/pr0607031. Epub 2007 Apr 19.
The c-subunit of ATP synthase (AtpH) is an 8 kD integral membrane protein with two transmembrane domains; we set out to demonstrate it amenable to top-down electrospray-ionization Fourier-transform mass spectrometry (FT-MS) using both collision activated and electron capture dissociation (CAD/ECD). Thermal activation concomitant with electron delivery was necessary for efficient ECD (activated-ion ECD; aiECD), yielding complementary information and greater sequence coverage in the transmembrane domains in comparison with CAD.
ATP合酶的c亚基（AtpH）是一种8 kD的整合膜蛋白，具有两个跨膜结构域；我们着手证明它适用于使用碰撞激活和电子捕获解离（CAD/ECD）的自上而下电喷雾电离傅里叶变换质谱（FT-MS）。与电子传递相伴的热激活对于高效ECD（激活离子ECD；aiECD）是必要的，与CAD相比，在跨膜结构域中产生了互补信息和更大的序列覆盖范围。
