Reynolds A M, Surks M I, Shapiro L E
Department of Medicine, Montefiore Medical Center, Bronx, New York 10467.
J Cell Physiol. 1991 Dec;149(3):544-7. doi: 10.1002/jcp.1041490325.
We have characterized the effects of supraphysiological concentrations of T3 on GC cells, a cultured cell line in which physiologic concentrations of T3 regulate cell growth, protein content, and growth hormone (GH) production. GC cells were exposed to 3 times (1.0 nM) and 80 times (25.0 nM) the physiologic concentration of T3 (0.3 nM) for either 4 d or for greater than 3 months. Both short and prolonged exposure to supranormal T3 concentrations supported maximal cell growth rate and induced significant increases in total protein (P less than 0.025) and GH production (P less than 0.01) per cell when compared to measurements in control GC cells. In addition, exposure to 1.0 nM and 25.0 nM T3 for greater than 3 months enhanced the toxicity of heat shock in a manner similar to previously described effects on GC cells due to T3 exposure of shorter duration. Thus, initial responses to raised T3 concentrations in cultured GC cells persisted without alteration when hormone exposure was prolonged for greater than 3 months.
我们已经研究了超生理浓度的T3对GC细胞的影响，GC细胞是一种培养细胞系，其中生理浓度的T3可调节细胞生长、蛋白质含量和生长激素（GH）的产生。将GC细胞暴露于3倍（1.0 nM）和80倍（25.0 nM）生理浓度的T3（0.3 nM）下，持续4天或超过3个月。与对照GC细胞的测量结果相比，短期和长期暴露于超正常T3浓度均支持最大细胞生长速率，并导致每个细胞的总蛋白（P<0.025）和GH产生显著增加（P<0.01）。此外，暴露于1.0 nM和25.0 nM T3超过3个月会增强热休克的毒性，其方式类似于先前描述的由于较短时间暴露于T3而对GC细胞产生的影响。因此，当激素暴露延长超过3个月时，培养的GC细胞中对升高的T3浓度的初始反应持续不变。
