Natalini Emanuela, Scortichini Marco
C.R.A. Istituto Sperimentale per la Frutticoltura, Roma, Italy.
FEMS Microbiol Lett. 2007 Jun;271(2):274-80. doi: 10.1111/j.1574-6968.2007.00725.x. Epub 2007 Apr 18.
The 16S-23S rRNA gene internal transcribed spacer region (ITS1) from 34 strains of Pseudomonas avellanae and some strains of Pseudomonas syringae pathovars was amplified and assessed by restriction fragment length polymorphism (RFLP) using 10 restriction enzymes. In addition, the ITS1 region of four representative P. avellanae strains was sequenced and compared by the neighbour-joining algorithm with that of P. syringae pathovars. Two main groups of P. avellanae strains were observed that did not correlate with their origin. The ITS1 region sequencing revealed a high similarity with the P. syringae complex. One group of P. avellanae strains showed high similarity to P. s. pv. actinidiae and P. s. pv. tomato; another group showed similarity with P. s. pv. tabaci and P. s. pv. glycinea. Two strains clustered with P. s. pv. pisi. The difficulties to unambiguously classify the strains associated with hazelnut decline in Greece and Italy are discussed.
对34株榛假单胞菌以及丁香假单胞菌部分致病变种菌株的16S - 23S rRNA基因内部转录间隔区（ITS1）进行了扩增，并使用10种限制性内切酶通过限制性片段长度多态性（RFLP）进行评估。此外，对4株代表性榛假单胞菌菌株的ITS1区域进行了测序，并通过邻接法算法与丁香假单胞菌致病变种的ITS1区域进行比较。观察到榛假单胞菌菌株分为两个主要组，这与它们的来源无关。ITS1区域测序显示与丁香假单胞菌复合体高度相似。一组榛假单胞菌菌株与猕猴桃丁香假单胞菌和番茄丁香假单胞菌高度相似；另一组与烟草丁香假单胞菌和大豆丁香假单胞菌相似。两株菌株与豌豆丁香假单胞菌聚类。讨论了对希腊和意大利与榛树衰退相关菌株进行明确分类的困难。
