Biology Centre of the Academy of Sciences of the Czech Republic, v.v.i., Institute of Plant Molecular Biology, Branisovská 31/1160, 370 05 Ceské Budejovice, Czech Republic.
J Microbiol Methods. 2010 Jul;82(1):90-4. doi: 10.1016/j.mimet.2010.05.004. Epub 2010 May 12.
The 16S-23S ribosomal internal transcribed spacer (ITS1) is often used as a subspecies or strain-specific molecular marker for various kinds of bacteria. However, the presence of different copies of ITS1 within a single genome has been reported. Such mosaicism may influence correct typing of many bacteria and therefore knowledge about exact configuration of this region in a particular genome is essential. In order to screen the variability of ITS1 among and within Pseudomonas syringae genomes, an oligonucleotide microarray targeting different configurations of ITS1 was developed. The microarray revealed seven distinct variants in 13 pathovars tested and detected mosaicism within the genomes of P. syringae pv. coronafaciens, pisi, syringae and tabaci. In addition, the findings presented here challenge the using of rRNA analysis for pathovar and strain determination.
16S-23S 核糖体内部转录间隔区（ITS1）常用于作为各种细菌的亚种或菌株特异性分子标记。然而，在单个基因组内存在不同拷贝的 ITS1 已被报道。这种镶嵌现象可能会影响许多细菌的正确分型，因此了解该区域在特定基因组中的准确结构至关重要。为了筛选丁香假单胞菌基因组中 ITS1 的多样性，开发了一种针对 ITS1 不同构型的寡核苷酸微阵列。微阵列在测试的 13 个致病变种中发现了 7 个不同的变体，并检测到丁香假单胞菌 pv. coronafaciens、pisi、syringae 和 tabaci 基因组内的镶嵌现象。此外，本研究结果对使用 rRNA 分析进行致病变种和菌株确定提出了挑战。
