使用地高辛标记的M13单链DNA探针检测人细小病毒B19病毒血症。

Use of M13 single-stranded DNA digoxigenin labelled probe for detection of human parvovirus B19 viraemia.

作者信息

Prato C, Paper T, Morinet F

机构信息

Centro de Diagnostico French, Buenos Aires, Republica Argentina.

出版信息

J Virol Methods. 1991 Oct;34(3):227-31. doi: 10.1016/0166-0934(91)90102-6.

DOI:10.1016/0166-0934(91)90102-6

PMID:1744217

Abstract

A dot-blot hybridization assay for serum B19 DNA is described using a non-radioactive (digoxigenin) M13 single-stranded DNA probe. The assay is sensitive (0.3 pg of B19 DNA) and has several advantages over similar assays which use radioactive or non-radioactive labelled plasmid probe for the routine detection of B19 DNA in sera.

摘要

描述了一种使用非放射性（地高辛配基）M13单链DNA探针进行血清B19 DNA斑点杂交检测的方法。该检测方法灵敏（可检测到0.3 pg的B19 DNA），与使用放射性或非放射性标记质粒探针常规检测血清中B19 DNA的类似检测方法相比，具有多个优点。