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用于检测人血清中B19细小病毒DNA的化学发光斑点杂交分析

Chemiluminescence dot blot hybridization assay for detection of B19 parvovirus DNA in human sera.

作者信息

Musiani M, Zerbini M, Gibellini D, Gentilomi G, Venturoli S, Gallinella G, Ferri E, Girotti S

机构信息

Istituto di Microbiologia, Universitá di Bologna, Italy.

出版信息

J Clin Microbiol. 1991 Sep;29(9):2047-50. doi: 10.1128/jcm.29.9.2047-2050.1991.

DOI:10.1128/jcm.29.9.2047-2050.1991
PMID:1774333
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC270258/
Abstract

A chemiluminescence dot blot hybridization assay was used for the detection of B19 parvovirus DNA in human sera by using digoxigenin-labeled probes. The probes were revealed immunoenzymatically by use of anti-digoxigenin Fab fragments conjugated with alkaline phosphatase. The chemiluminescence signal was obtained by reacting the labeled probe-target complex with an enzyme-triggerable dioxetane substrate. The emitted photons were detected with instant photographic films. In the search for B19 parvovirus DNA, 2,808 serum samples were analyzed.

摘要

采用化学发光斑点杂交分析方法，使用地高辛标记的探针检测人血清中的B19细小病毒DNA。通过使用与碱性磷酸酶偶联的抗地高辛Fab片段，以免疫酶法显示探针。通过使标记的探针 - 靶标复合物与酶触发的二氧杂环丁烷底物反应获得化学发光信号。用即时摄影胶片检测发射的光子。在对B19细小病毒DNA的检测中，分析了2808份血清样本。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de05/270258/82eec25f5cf8/jcm00045-0316-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de05/270258/eeddc124aa5c/jcm00045-0316-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de05/270258/82eec25f5cf8/jcm00045-0316-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de05/270258/eeddc124aa5c/jcm00045-0316-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de05/270258/82eec25f5cf8/jcm00045-0316-b.jpg

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本文引用的文献

1
Suppression of endogenous avidin-binding activity in tissues and its relevance to biotin-avidin detection systems.组织中内源性抗生物素蛋白结合活性的抑制及其与生物素-抗生物素蛋白检测系统的相关性。
J Histochem Cytochem. 1981 Oct;29(10):1196-204. doi: 10.1177/29.10.7028859.
2
Diagnostic assays with monoclonal antibodies for the human serum parvovirus-like virus (SPLV).用于人类血清细小病毒样病毒（SPLV）的单克隆抗体诊断检测法。
J Hyg (Lond). 1983 Aug;91(1):113-30. doi: 10.1017/s0022172400060095.
3
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
J Clin Microbiol. 1996 May;34(5):1313-6. doi: 10.1128/jcm.34.5.1313-1316.1996.
4
Chemiluminescent in situ hybridization for the detection of cytomegalovirus DNA.用于检测巨细胞病毒DNA的化学发光原位杂交技术
Am J Pathol. 1996 Apr;148(4):1105-12.
5
Detection of parvovirus B19 in donated blood: a model system for screening by polymerase chain reaction.献血中细小病毒B19的检测：一种通过聚合酶链反应进行筛查的模型系统。
J Clin Microbiol. 1993 Feb;31(2):323-8. doi: 10.1128/jcm.31.2.323-328.1993.
6
Evaluation of polymerase chain reaction for diagnosis of pneumococcal pneumonia.聚合酶链反应用于诊断肺炎球菌肺炎的评估。
J Clin Microbiol. 1993 Oct;31(10):2661-6. doi: 10.1128/jcm.31.10.2661-2666.1993.
7
WIN 54954 treatment of mice infected with a diabetogenic strain of group B coxsackievirus.
Antimicrob Agents Chemother. 1993 Aug;37(8):1593-8. doi: 10.1128/AAC.37.8.1593.
8
Different syndromes associated with B19 parvovirus viraemia in paediatric patients: report of four cases.小儿患者中与B19细小病毒血症相关的不同综合征：4例报告
Eur J Pediatr. 1992 Nov;151(11):815-7. doi: 10.1007/BF01957931.
一种将DNA限制性内切酶片段放射性标记至高比活度的技术。
Anal Biochem. 1983 Jul 1;132(1):6-13. doi: 10.1016/0003-2697(83)90418-9.
4
Endogenous avidin-binding activity in human lymphoid tissue.人淋巴组织中的内源性抗生物素蛋白结合活性。
J Clin Pathol. 1984 Feb;37(2):223-5. doi: 10.1136/jcp.37.2.223.
5
Intrauterine parvovirus infection associated with hydrops fetalis.与胎儿水肿相关的宫内细小病毒感染。
Lancet. 1984 Nov 3;2(8410):1033-4. doi: 10.1016/s0140-6736(84)91126-7.
6
Outbreak of aplastic crises in sickle cell anaemia associated with parvovirus-like agent.与细小病毒样病原体相关的镰状细胞贫血再生障碍危象暴发。
Lancet. 1981 Sep 19;2(8247):595-7. doi: 10.1016/s0140-6736(81)92739-2.
7
Detection of human parvovirus using a molecularly cloned probe.
J Med Virol. 1985 Feb;15(2):173-81. doi: 10.1002/jmv.1890150210.
8
Human parvovirus infection in pregnancy and hydrops fetalis.妊娠期间的人细小病毒感染与胎儿水肿
N Engl J Med. 1987 Jan 22;316(4):183-6. doi: 10.1056/NEJM198701223160403.
9
Human parvovirus infection in early rheumatoid and inflammatory arthritis.类风湿性关节炎和炎症性关节炎早期的人细小病毒感染
Ann Rheum Dis. 1986 Oct;45(10):832-8. doi: 10.1136/ard.45.10.832.
10
Human parvovirus B19-induced epidemic acute red cell aplasia in patients with hereditary hemolytic anemia.人细小病毒B19在遗传性溶血性贫血患者中引发的流行性急性红细胞再生障碍。
Blood. 1986 May;67(5):1411-7.