[不明原因复发性自然流产患者蜕膜中转录因子GATA-3和T-bet mRNA表达的研究]

[Study on the expression of transcription factor GATA-3 and T-bet mRNA in decidua of women with unexplained recurrent spontaneous abortion].

作者信息

Qiu Li-Hua, Lin Qi-de

机构信息

Department of Obstetrics and Gynecology, Affiliated Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200001, China.

出版信息

Zhonghua Fu Chan Ke Za Zhi. 2007 Feb;42(2):96-8.

PMID:17442182

Abstract

OBJECTIVE

To investigate the roles of transcription factor GATA-3 and T-bet at the fetal-maternal interface in the pathogenesis of unexplained recurrent spontaneous abortion (URSA).

METHODS

The expression of GATA-3 and T-bet mRNA was examined by in situ hybridization. Decidua was obtained from 20 women with URSA and 20 normal pregnant (NP) women.

RESULTS

(1) The number of GATA-3 positive cells per high power field in women with URSA (25 +/- 16) was significantly lower than those in NP women (38 +/- 16) (P < 0.05). (2) The number of T-bet positive cells per high power field in women with URSA (59 +/- 17) was significantly higher than those in NP women (46 +/- 18) (P < 0.05). (3) Numbers of cells expressing GATA-3 mRNA correlated significantly with T-bet mRNA positive cell numbers in decidua (r = -0.55, P < 0.01).

CONCLUSIONS

The results demonstrate that upregulated expression of T-bet and downregulated expression of GATA-3 are found in the decidua of women with URSA. Imbalance of GATA-3/T-bet transcription factors might interfere with type 1 helper T cell (Th1)/type 2 helper T cell (Th2) differentiation at the fetal-maternal interface and cause URSA.

摘要

目的

探讨转录因子GATA-3和T-bet在母胎界面处不明原因复发性自然流产（URSA）发病机制中的作用。

方法

采用原位杂交法检测GATA-3和T-bet mRNA的表达。从20例URSA患者和20例正常孕妇（NP）获取蜕膜。

结果

（1）URSA患者每高倍视野中GATA-3阳性细胞数（25±16）显著低于NP组（38±16）（P<0.05）。（2）URSA患者每高倍视野中T-bet阳性细胞数（59±17）显著高于NP组（46±18）（P<0.05）。（3）蜕膜中表达GATA-3 mRNA的细胞数与T-bet mRNA阳性细胞数显著相关（r=-0.55，P<0.01）。