Müller Frank A, Bottino Marco C, Müller Lenka, Henriques Vinicius A R, Lohbauer Ulrich, Bressiani Ana Helena A, Bressiani José C
Department of Materials Science (III)-Biomaterials, University of Erlangen-Nuernberg, Erlangen, Germany.
Dent Mater. 2008 Jan;24(1):50-6. doi: 10.1016/j.dental.2007.02.005. Epub 2007 Apr 17.
Titanium alloys are considered the material of choice when used as endosteal part of implants. However, they are not able to bond directly to bone. The objective of this study was to suggest a chemical surface treatment for Ti-13Nb-13Zr to initiate the formation of hydroxy carbonated apatite (HCA) during in vitro bioactivity tests in simulated body fluid (SBF).
Titanium, niobium, and zirconium hydride powders were blended, compacted and sintered. Sintered Ti-13Nb-13Zr samples were etched in HCl, H(3)PO(4), and in a mixture of HF+HNO(3), respectively, and subsequently pretreated in NaOH. The influence of acid etching conditions on the microstructure of the Ti-13Nb-13Zr alloys as well as on the rate of HCA formation was evaluated using SEM-EDS, FTIR, and gravimetric analyses.
Sintered Ti-13Nb-13Zr alloys consist of a Widmannstätten (alpha+beta) microstructure. Exposure of chemically etched and NaOH activated samples to SBF for 1 week leads to the formation of a HCA layer on the surface of HCl as well as H(3)PO(4) treated samples. No HCA formation was found on HNO(3) treated samples. After 2 weeks in SBF the mass increase, that can be correlated to the HCA formation rate, was the highest for HCl pretreated samples (2.4 mg/cm(2)) followed by H(3)PO(4) (0.8 mg/cm(2)) and HNO(3) pretreated ones (0.2 mg/cm(2)).
Since the in vitro HCA formation from SBF is generally accepted as a typical feature for bioactive materials, it is supposed that HCl etching with subsequent NaOH treatment might enhance the in vivo bone-bonding ability of Ti-13Nb-13Zr.
钛合金被认为是用作植入物骨内部分的首选材料。然而，它们无法直接与骨结合。本研究的目的是提出一种针对Ti-13Nb-13Zr的化学表面处理方法，以在模拟体液（SBF）中的体外生物活性测试期间引发羟基碳酸磷灰石（HCA）的形成。
将钛、铌和氢化锆粉末混合、压实并烧结。烧结后的Ti-13Nb-13Zr样品分别在HCl、H₃PO₄以及HF + HNO₃的混合物中蚀刻，随后在NaOH中进行预处理。使用扫描电子显微镜-能谱仪（SEM-EDS）、傅里叶变换红外光谱仪（FTIR）和重量分析评估酸蚀刻条件对Ti-13Nb-13Zr合金微观结构以及HCA形成速率的影响。
烧结后的Ti-13Nb-13Zr合金具有魏德曼组织（α+β）微观结构。经过化学蚀刻和NaOH活化的样品在SBF中暴露1周后，HCl以及H₃PO₄处理的样品表面会形成HCA层。在HNO₃处理的样品上未发现HCA形成。在SBF中放置2周后，与HCA形成速率相关的质量增加量，HCl预处理的样品最高（2.4 mg/cm²），其次是H₃PO₄处理的样品（0.8 mg/cm²）和HNO₃预处理的样品（0.2 mg/cm²）。
由于从SBF中体外形成HCA通常被认为是生物活性材料的典型特征，因此推测随后进行NaOH处理的HCl蚀刻可能会增强Ti-13Nb-13Zr在体内的骨结合能力。
