Papini Sandra, Rosellini Alfredo, De Matteis Anna, Campani Daniela, Selli Cesare, Caporali Andrea, Bettuzzi Saverio, Revoltella Roberto P
Institute of Biomedical Technologies, National Research Council of Italy, Pisa, Italy.
Pathol Res Pract. 2007;203(4):209-16. doi: 10.1016/j.prp.2007.02.004.
Human prostatic adenocarcinoma fragments (1-6mm) were cultured on collagen sponges in medium supplemented or not supplemented with 4,5alpha-dihydrotesterone (DHT) until 3 weeks, maintaining the three-dimensional (3D) epithelial and stromal organization present in the tumor in vivo. With time, in the presence of DHT, locally progressive cribriform nests of neoplastic cells with proliferative rates higher than those inside the fragment developed on the surface, while the stroma became more dissociated, and fibrosis replaced the muscular component. The 3D-culture provides a promising approach for studying the development and phenotype of prostate epithelial tumor progenitor cells and their stromal interactions.
将人前列腺腺癌组织碎片（1 - 6毫米）接种于胶原海绵上，在添加或不添加4,5α - 双氢睾酮（DHT）的培养基中培养3周，以维持肿瘤在体内呈现的三维（3D）上皮和基质组织结构。随着时间推移，在DHT存在的情况下，表面会形成局部进展性的筛状巢状肿瘤细胞，其增殖速率高于组织碎片内部的细胞，同时基质变得更加离散，纤维化取代了肌肉成分。这种三维培养为研究前列腺上皮肿瘤祖细胞的发育、表型及其与基质的相互作用提供了一种有前景的方法。
