Papini Sandra, Rosellini Alfredo, Campani Daniela, DeMatteis Anna, Selli Cesare, Revoltella Roberto Paolo
Institute of Biomedical Technologies, Immunobiology and Cell Differentiation Unit, National Research Council of Italy, Pisa, Italy.
Prostate. 2004 Jun 1;59(4):383-92. doi: 10.1002/pros.20021.
A three-dimensional organotypic culture method has been developed for selectively growing epithelial basal cells from human benign prostate.
Tissue fragments were cultured on sponges for several weeks and the viability of luminal and basal epithelium and cellular responses to 4,5alpha-dihydrotesterone (DHT) stimulation were studied.
The gland tissue could be successfully maintained showing preservation of ducts and lobules as in vivo. Without DHT, a progressive hyperplasia of basal cells was observed: these cells proliferated with retention of the lumen or forming nests with squamous differentiation, lining the surface of the fragment and migrating to the underlying sponge. In contrast, secretory cells disappeared. Epithelial cells isolated from long-term cultures showed a typical basal cell-immunophenotype. DHT-treated tissues maintained a much higher percentage of luminal cells than untreated tissues.
These systems allow the study of proliferation and differentiation of basal cells within their natural microenvironment as well as prostate pathobiology.
已开发出一种三维器官型培养方法，用于从人良性前列腺中选择性培养上皮基底细胞。
将组织碎片在海绵上培养数周，研究管腔上皮和基底上皮的活力以及细胞对4,5α-二氢睾酮（DHT）刺激的反应。
腺体组织能够成功维持，显示出与体内一样的导管和小叶保存。在没有DHT的情况下，观察到基底细胞进行性增生：这些细胞增殖，保留管腔或形成具有鳞状分化的巢，排列在碎片表面并迁移到下面的海绵中。相比之下，分泌细胞消失。从长期培养物中分离出的上皮细胞显示出典型的基底细胞免疫表型。DHT处理的组织比未处理的组织维持更高比例的管腔细胞。
这些系统允许在其自然微环境中研究基底细胞的增殖和分化以及前列腺病理生物学。
