Chen Yu-Ping, Qiao Yuan-Yuan, Zhao Xiao-Hang, Chen Hong-Song, Wang Yan, Wang Zhuozhi
Department of Dermatology, Xijing Hospital, The Fourth Military Medical University, Xi'an, China.
Clin Vaccine Immunol. 2007 Jun;14(6):720-5. doi: 10.1128/CVI.00310-06. Epub 2007 Apr 18.
Bispecific antibodies have immense potential for use in clinical applications. In the present study, a bispecific diabody against human red blood cells (RBCs) and hepatitis B virus surface antigen (HBsAg) was used to detect HBsAg in blood specimens. The bispecific diabody was constructed by crossing over the variable region of the heavy chains and the light chains of anti-RBC and anti-HBsAg antibodies with a short linker, SRGGGS. In enzyme-linked immunosorbent assays, this bispecific diabody showed specific binding to both RBCs and HBsAg. When this bispecific diabody was mixed with human blood specimens in the presence of HBsAg, the dual binding sites of the diabody caused agglutination of human RBCs. This diabody-mediated agglutination assay was then used to test 712 clinical blood specimens and showed 97.7% sensitivity and 100% specificity when the results were compared with those of the conventional immunoassay, which was used as a reference. This autologous RBC agglutination assay provides a simple approach for rapid screening for HBsAg in blood specimens.
双特异性抗体在临床应用中具有巨大潜力。在本研究中，一种针对人红细胞（RBC）和乙型肝炎病毒表面抗原（HBsAg）的双特异性双体被用于检测血液标本中的HBsAg。该双特异性双体通过将抗RBC和抗HBsAg抗体的重链可变区和轻链可变区用短接头SRGGGS进行交叉连接构建而成。在酶联免疫吸附试验中，这种双特异性双体显示出与RBC和HBsAg均有特异性结合。当这种双特异性双体在有HBsAg存在的情况下与人血液标本混合时，双体的双结合位点会导致人RBC发生凝集。然后，这种双体介导的凝集试验被用于检测712份临床血液标本，与作为参考的传统免疫测定结果相比，其灵敏度为97.7%，特异性为100%。这种自体RBC凝集试验为血液标本中HBsAg的快速筛查提供了一种简单方法。
