Rasheed Muzib Hassanal, Beevi Syed Sultan, Rajaraman R, Bose S J C
Department of Surgery, Maryborough Base Hospital, Maryborough, Queensland, Australia.
J Surg Oncol. 2007 Sep 1;96(3):194-9. doi: 10.1002/jso.20818.
Oxidative stress by reactive oxygen species (ROS) and nitrosative stress by reactive nitrogen species (RNS) are proven initiators and promoters in carcinogenesis. Elevated ROS/RNS with lowered antioxidants occur in patients with squamous cell carcinoma of oral cavity. Ours is the first study to evaluate the effect of curative resection on both oxidative and nitrosative stress in such patients.
This study was conducted on 24 cancer patients and with age- and sex-matched healthy controls. Lipid peroxidation products, nitric oxide (NO) products and ceruloplasmin (CPL) in plasma were measured before and after surgery. Similarly enzymatic antioxidants in erythrocytes and non-enzymatic antioxidants in plasma were measured.
Statistically significant elevation of lipid peroxidation, NO products and CPL and depletion of antioxidants were found in cancer patients compared with controls. After curative surgical resection there was a statistically significant fall in oxidants and CPL coupled with a rise in antioxidants.
Our results suggest that oxidative/nitrosative stress could play a significant role in oral cavity cancer (OCC) and that curative resection is effective in alleviating this oxidative/nitrosative burden. Significant mitigation of oxidative/nitrosative stress could indicate the completeness of resection since tumor forms the major source of oxidants.
活性氧（ROS）引起的氧化应激和活性氮（RNS）引起的亚硝化应激已被证实是致癌作用的起始因素和促进因素。口腔鳞状细胞癌患者体内ROS/RNS升高而抗氧化剂水平降低。我们的研究是首个评估根治性切除对此类患者氧化应激和亚硝化应激影响的研究。
本研究纳入了24例癌症患者，并设置了年龄和性别匹配的健康对照。在手术前后测量血浆中的脂质过氧化产物、一氧化氮（NO）产物和铜蓝蛋白（CPL）。同样，也测量了红细胞中的酶促抗氧化剂和血浆中的非酶促抗氧化剂。
与对照组相比，癌症患者的脂质过氧化、NO产物和CPL在统计学上显著升高，抗氧化剂水平降低。根治性手术切除后，氧化剂和CPL在统计学上显著下降，同时抗氧化剂水平升高。
我们的结果表明，氧化/亚硝化应激可能在口腔癌（OCC）中起重要作用，根治性切除可有效减轻这种氧化/亚硝化负担。氧化/亚硝化应激的显著减轻可能表明切除的彻底性，因为肿瘤是氧化剂的主要来源。
