Beevi Syed Sultan, Rasheed Muzib Hassanal, Geetha Arumugam
Department of Physiology and Biochemistry, Ragas Dental College and Hospital, Chennai-600 119, Tamil Nadu, India.
Clin Chim Acta. 2007 Jan;375(1-2):119-23. doi: 10.1016/j.cca.2006.06.028. Epub 2006 Jun 29.
We conducted a study to evaluate reactive oxygen species (ROS) and reactive nitrogen species (RNS) simultaneously together with the antioxidant status in patients with cervical carcinoma.
We measured lipid peroxidation product, including malondialdehye (MDA), nitric oxide (NO) products, including nitrite (NO(2)(-)), nitrate (NO(3)(-)) and total nitrite (TNO(2)(-)) and antioxidant enzymes in 45 patients with cervical cancer and compared them against 30 healthy controls.
Plasma as well as erythrocyte MDA and plasma NO levels was higher (p<0.001) in cervical cancer as compared to healthy controls. Antioxidant enzymes, Superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activities, were decreased (p<0.001) whereas glutathione S-transferase (GST) activity was increased (p<0.001) in cervical cancer patients. Lipid peroxidation and NO products accumulation correlated significantly with a deranged antioxidant system.
We demonstrated a possible involvement of both oxidative and nitrosative stress, as evidenced by increased lipid peroxidation and NO levels with altered antioxidant defense system in the pathogenesis of cervical cancer.
我们开展了一项研究，以同时评估宫颈癌患者体内的活性氧（ROS）和活性氮（RNS）以及抗氧化状态。
我们检测了45例宫颈癌患者的脂质过氧化产物，包括丙二醛（MDA）、一氧化氮（NO）产物，包括亚硝酸盐（NO₂⁻）、硝酸盐（NO₃⁻）和总亚硝酸盐（TNO₂⁻）以及抗氧化酶，并将其与30名健康对照者进行比较。
与健康对照者相比，宫颈癌患者的血浆以及红细胞MDA和血浆NO水平更高（p<0.001）。宫颈癌患者的抗氧化酶，超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPx）活性降低（p<0.001），而谷胱甘肽S-转移酶（GST）活性升高（p<0.001）。脂质过氧化和NO产物积累与紊乱的抗氧化系统显著相关。
我们证明了氧化应激和亚硝化应激可能均参与其中，宫颈癌发病机制中脂质过氧化增加和NO水平升高以及抗氧化防御系统改变即为证据。
