Xin Ming-xiu, Zhou Pei-jin
College of Life Sciences, Beijing Normal University, Beijing 100875, China.
J Zhejiang Univ Sci B. 2007 Apr;8(4):260-5. doi: 10.1631/jzus.2007.B0260.
The yeast strain (Y18) was isolated from a soil sample collected from Fildes Peninsula, Antarctica. The strain is a psychrophilic yeast with optimum and maximum growth temperatures of 10 degrees C and 18 degrees C, respectively. Teliospores were formed after 7 d on malt agar, when the germination of teliospores was observed. Both inositol and D-glucuronate were assimilated. Positive results of the DBB (diazonium blue B) color reaction, urease test, and starch formation were observed. The major CoQ is Q(8). All results indicated that Y18 belongs to the genes of Mrakia. The 18S rDNA sequence analyses showed that Y18 is closely related to Mrakia frigida. DNA-DNA relatedness study, and some biochemistry characteristics indicated that Y18 represents a new species for which Mrakia psychrophila sp. nov. is proposed.
酵母菌株(Y18)是从南极菲尔德斯半岛采集的土壤样本中分离出来的。该菌株是一种嗜冷酵母,其最适生长温度和最高生长温度分别为10℃和18℃。在麦芽琼脂上培养7天后形成冬孢子,此时观察到冬孢子的萌发。肌醇和D-葡萄糖醛酸均可被同化。观察到DBB(重氮蓝B)颜色反应、脲酶试验和淀粉形成的阳性结果。主要辅酶Q为Q(8)。所有结果表明Y18属于Mrakia属。18S rDNA序列分析表明Y18与嗜冷Mrakia密切相关。DNA-DNA相关性研究及一些生化特性表明Y18代表一个新物种,为此提出嗜冷Mrakia新种。