Oudshoorn M, Horn P A, Tilanus M, Yu N
Europdonor Foundation, Leiden, The Netherlands.
Tissue Antigens. 2007 Apr;69 Suppl 1:10-2. doi: 10.1111/j.1399-0039.2006.758_5.x.
The goal was to establish the best typing techniques and strategies for donors selected as potential donors for a specific patient. Complete sequence of exons 2 and 3 for class I and exon 2 for class II molecules by sequencing-based typing was deemed the best technique in reducing problems with ambiguities and null alleles as well as the possibility to establish a completely automated system. Focusing on multiple approaches for typing was regarded as a better strategy than continued usage of serological methods although the latter technique was still considered to be useful by some for detection of null alleles. The patient and finally the selected donor, including the back-up donor, should be typed for at the highest level and typing ambiguities should be resolved. In very difficult cases, race, haplotype information and allele frequency in the population should help to decide whether or not to resolve the ambiguity. Cellular techniques such as the cytotoxic T lymphocyte precursor test may help to choose between single class I mismatched donors in experienced laboratories only.
目标是为被选定作为特定患者潜在供体的供体建立最佳的分型技术和策略。通过基于测序的分型方法对I类分子的外显子2和3以及II类分子的外显子2进行完整测序，被认为是减少模糊性和无效等位基因问题以及建立完全自动化系统可能性的最佳技术。尽管一些人仍认为血清学方法对检测无效等位基因有用，但专注于多种分型方法被视为比继续使用血清学方法更好的策略。患者以及最终选定的供体，包括备用供体，都应进行最高水平的分型，分型模糊性应得到解决。在非常困难的情况下，种族、单倍型信息和人群中的等位基因频率应有助于决定是否解决模糊性问题。细胞技术，如细胞毒性T淋巴细胞前体试验，仅在经验丰富的实验室中可能有助于在单I类错配供体之间进行选择。
