de la Chica-Rodríguez Susana, Cortés-Denia Pedro, Ramírez-Expósito María Jesús, de Saavedra José Manuel Arias, Sánchez-Agesta Rafael, Pérez María del Cármen, Martínez-Martos José Manuel
Experimental and Clinical Physiopathology Research Group, Department of Health Sciences, Faculty of Experimental and Health Sciences, University of Jaén, Jaén, Spain.
Fertil Steril. 2007 Oct;88(4 Suppl):1071-5. doi: 10.1016/j.fertnstert.2006.12.021. Epub 2007 Apr 18.
To analyze the role of the local renin-angiotensin system (RAS) in the female reproductive system to modulate ovarian steroidogenesis and its relationship with alpha(1)adrenergic receptors.
Observational study.
University laboratory.
ANIMAL(S): Adult female Wistar rats treated with doxazosin (10 mg/kg) or vehicle for 15 days.
INTERVENTION(S): Samples from the whole right ovary were dissected after perfusion with saline. The soluble and membrane-bound fractions were obtained from these samples. Also, blood samples were used to obtain the serum.
MAIN OUTCOME MEASURE(S): Fluorometric measurement of soluble and membrane-bound RAS-regulating proteolytic regulatory enzyme activities by using arylamide derivatives as substrates. Time-resolved fluoroimmunoassay of serum E(2) and P.
RESULT(S): alpha(1)Adrenergic receptor blockade increases ovarian soluble and membrane-bound aminopeptidase A and decreases membrane-bound aminopeptidase N and aminopeptidase B. Furthermore, serum P levels increased, whereas serum E(2) did not change.
CONCLUSION(S): Ovarian P production, at least in the rat, is regulated by noradrenaline through a mechanism of action in which the RAS is involved, with a main role for angiotensin III.
