Bruen Trevor C, Poss Mary
McGill Center for Bioinformatics, McGill University, Montreal, Canada.
Virology. 2007 Aug 1;364(2):362-70. doi: 10.1016/j.virol.2007.03.023. Epub 2007 Apr 18.
Recombination contributes significantly to diversity within virus populations and ultimately to viral evolution. Here we use a recently developed statistical test to perform exploratory analysis of recombination in fourteen feline immunodeficiency virus (FIVpco) genomes derived from a wild population of cougars. We use both the global and local Phi statistical test as an overall guide to predict where recombination may have occurred. Further analyses, including similarity plots and phylogenetic incongruence tests, confirmed that three FIVpco lineages were derived from recombinant events. Interestingly, the regions of mosaic origin were clustered in the area encoding lentiviral accessory genes and largely spared the viral structural genes. Because some of the mosaic strains are currently geographically disparate, our data indicate that the dispersal of cougars infected with these strains was preceded by recombination events. These results suggest that recombination has played an important role in the evolution of FIVpco for this wild population of cougars.
重组对病毒群体内的多样性有显著贡献，并最终推动病毒进化。在此，我们使用一种最近开发的统计测试方法，对源自野生美洲狮群体的14个猫免疫缺陷病毒（FIVpco）基因组中的重组进行探索性分析。我们使用全局和局部Phi统计测试作为预测重组可能发生位置的总体指南。进一步的分析，包括相似性图和系统发育不一致性测试，证实了三个FIVpco谱系源自重组事件。有趣的是，嵌合起源区域聚集在编码慢病毒辅助基因的区域，而病毒结构基因基本未受影响。由于一些嵌合菌株目前在地理上分布不同，我们的数据表明，感染这些菌株的美洲狮的扩散发生在重组事件之前。这些结果表明，重组在这个野生美洲狮群体的FIVpco进化中发挥了重要作用。
