Chen S P, Lee M C, Sun Y F, Cheng I C, Yang P C, Lin Y L, Jong M H, Robertson I D, Edwards J R, Ellis T M
Division of Animal Medicine, Animal Technology Institute Taiwan, Chunan, Miaoli, Taiwan.
Vaccine. 2007 May 30;25(22):4464-9. doi: 10.1016/j.vaccine.2007.03.019. Epub 2007 Mar 28.
The immune response to structural and non-structural proteins (NSPs) was studied on sequential serum samples in swine from O/Taiwan/97 FMDV challenge studies, outbreaks and after vaccination. The results showed that pigs vaccinated with a commercial vaccine prior to or after infection maintained high neutralizing antibody titers with gradual decline from peak titers over the duration of this study. However, neutralizing antibody titers in non-vaccinated pigs only reached moderate levels 2-4 weeks post infection and remained low thereafter. For the 3B and 3ABC NSP antibody ELISA responses, there were gradually decreasing levels of NSP antibody over time. In multiple vaccinations, all pigs showed significant increases in neutralizing antibodies after booster vaccination. For the 3B NSP antibody ELISA after vaccination, the mean S/P ratios for pigs vaccinated with all three FMD vaccines were all below the 0.23 cut-off value set by the manufacture, but some sera from individual vaccinated pigs gave results above this cut-off after primary or secondary vaccination. However, with the 3ABC NSP antibody ELISA, all sera from vaccinated pigs had negative results for NSP antibody for all time points.
在台湾地区O/97口蹄疫病毒攻毒试验、疫情爆发及疫苗接种后的猪群中，对连续采集的血清样本进行了针对结构蛋白和非结构蛋白（NSPs）的免疫反应研究。结果显示，在感染前或感染后接种商业疫苗的猪，在本研究期间中和抗体滴度维持在较高水平，并从峰值逐渐下降。然而，未接种疫苗的猪在感染后2 - 4周中和抗体滴度仅达到中等水平，之后一直维持在低水平。对于3B和3ABC NSP抗体ELISA反应，随着时间推移NSP抗体水平逐渐下降。在多次接种中，所有猪在加强免疫后中和抗体均显著增加。接种疫苗后进行3B NSP抗体ELISA检测，三种口蹄疫疫苗接种猪的平均S/P比值均低于厂家设定的0.23临界值，但部分接种猪个体的血清在初次或二次接种后结果高于该临界值。然而，采用3ABC NSP抗体ELISA检测时，接种猪所有时间点的血清NSP抗体结果均为阴性。
