Eblé P L, de Bruin M G M, Bouma A, van Hemert-Kluitenberg F, Dekker A
Central Institute for Animal Disease Control (CIDC-Lelystad), P.O. Box 2004, 8203 AA Lelystad, The Netherlands.
Vaccine. 2006 Feb 27;24(9):1274-81. doi: 10.1016/j.vaccine.2005.09.040. Epub 2005 Oct 4.
This study compares the immune responses and protection induced by intra-typic heterologous vaccination with that induced by homologous vaccination against challenge with foot-and-mouth disease virus (FMDV). Humoral and cell-mediated immune responses and protection against challenge with FMDV O Taiwan were examined in a non-vaccinated group, a group vaccinated with O Taiwan FMD vaccine and a group vaccinated with O Manisa FMD vaccine. Five pigs from each group were challenged with FMDV type O Taiwan 14 days after vaccination and five other pigs were contact-exposed to the inoculated pigs. Both homologous and heterologous vaccination protected against challenge with FMDV O Taiwan at 2 weeks after vaccination. In the heterologous vaccinated group, cross-neutralizing antibody titres against O Taiwan could be detected although the ratio 'r(1)' was 0.4, which was significantly smaller than the critical r-value. Cell-mediated immune responses were detected after both homologous and heterologous vaccination. Virus-induced in vitro lymphocyte (cross-) proliferation and production of both a Th1-type (IFN-gamma) and a Th2-type (IL-10) cytokine response were demonstrated in cultures of peripheral blood mononuclear cells (PBMC). The findings show that heterologous (emergency) vaccination can prevent clinical disease and shedding of virus. The induction of the cell-mediated immune responses after (heterologous) vaccination needs more research but data on these responses might provide additional tools for both vaccine choice and vaccine development.
本研究比较了口蹄疫病毒（FMDV）攻击时，同型异源疫苗接种与同源疫苗接种所诱导的免疫反应和保护作用。在一个未接种疫苗组、一个接种台湾O型口蹄疫疫苗组和一个接种马尼萨O型口蹄疫疫苗组中，检测了体液免疫和细胞介导的免疫反应以及针对台湾O型FMDV攻击的保护作用。每组五只猪在接种疫苗14天后用台湾O型FMDV攻击，另外五只猪与接种猪接触感染。同源和异源疫苗接种在接种后2周均能保护猪免受台湾O型FMDV的攻击。在异源疫苗接种组中，尽管“r(1)”比值为0.4，显著小于临界r值，但仍可检测到针对台湾O型的交叉中和抗体滴度。同源和异源疫苗接种后均检测到细胞介导的免疫反应。在外周血单核细胞（PBMC）培养中证实了病毒诱导的体外淋巴细胞（交叉）增殖以及Th1型（IFN-γ）和Th2型（IL-10）细胞因子反应的产生。研究结果表明，异源（紧急）疫苗接种可预防临床疾病和病毒排出。（异源）疫苗接种后细胞介导免疫反应的诱导需要更多研究，但这些反应的数据可能为疫苗选择和疫苗开发提供额外的工具。
