Secondo Agnese, Staiano Ilaria Rosaria, Scorziello Antonella, Sirabella Rossana, Boscia Francesca, Adornetto Annagrazia, Canzoniero Lorella Maria Teresa, Di Renzo Gianfranco, Annunziato Lucio
Division of Pharmacology, Department of Neuroscience, School of Medicine, Federico II University of Naples, Via Pansini 5, 80131 Naples, Italy.
Ann N Y Acad Sci. 2007 Mar;1099:481-5. doi: 10.1196/annals.1387.052.
Chemical hypoxia produces depletion of ATP, intracellular Ca2+ overload, and cell death. The role of Na+/Ca2+ exchanger (NCX), the major plasma membrane Ca2+ extruding system, has been explored in chemical hypoxia using BHK cells stably transfected with the three mammalian NCX isoforms: NCX1, NCX2, and NCX3. Here we report that the three isoforms show similar activity evaluated as [Ca2+]i increase evoked by Na+-free medium exposure in Fura-2-loaded single cells and NCX3 transfected cells are less vulnerable to chemical hypoxia compared to NCX1- and NCX2-transfected cells, suggesting that NCX3 could play a more relevant protective role during chemical hypoxia.
化学性缺氧会导致三磷酸腺苷（ATP）耗竭、细胞内钙离子过载及细胞死亡。钠钙交换体（NCX）是主要的质膜钙离子外排系统，利用稳定转染了三种哺乳动物NCX亚型（NCX1、NCX2和NCX3）的幼仓鼠肾（BHK）细胞，对其在化学性缺氧中的作用进行了研究。在此我们报告，在以Fura-2负载的单细胞中，通过无钠培养基暴露诱发的细胞内钙离子浓度（[Ca2+]i）升高来评估，这三种亚型表现出相似的活性，并且与转染NCX1和NCX2的细胞相比，转染NCX3的细胞对化学性缺氧的耐受性更强，这表明NCX3在化学性缺氧期间可能发挥更重要的保护作用。
