植物中总抗氧化能力的快速测定

Rapid measurement of total antioxidant capacity in plants.

作者信息

Gillespie Kelly M, Chae June M, Ainsworth Elizabeth A

机构信息

Program in Physiological and Molecular Plant Biology and Institute for Genomic Biology, University of Illinois Urbana-Champaign, 147 ERML, 1201 W. Gregory Drive, Urbana, Illinois 61801, USA.

出版信息

Nat Protoc. 2007;2(4):867-70. doi: 10.1038/nprot.2007.100.

DOI:10.1038/nprot.2007.100

PMID:17446887

Abstract

There is growing interest in measuring the antioxidant status of plant tissues. This protocol describes the oxygen radical absorbance capacity (ORAC) assay, which measures antioxidant inhibition of peroxyl radical-induced oxidations and is a measure of total antioxidant capacity. The assay is performed in a microplate and is assessed with a 96-well multi-detection plate reader. Total antioxidant capacity of 64 experimental samples can easily be analyzed in 1 d. This assay is presented along with rapid assays for total phenolic content and total ascorbate content. Overall, these assays provide a general diagnostic tool of the antioxidant capacity in leaf tissue extracts.

摘要

测量植物组织抗氧化状态的兴趣与日俱增。本方案描述了氧自由基吸收能力（ORAC）测定法，该方法用于测量抗氧化剂对过氧自由基诱导氧化的抑制作用，是总抗氧化能力的一种度量。该测定在微孔板中进行，并使用96孔多检测微孔板读数器进行评估。64个实验样品的总抗氧化能力可在1天内轻松分析完毕。本测定法以及总酚含量和总抗坏血酸含量的快速测定法一并给出。总体而言，这些测定法为叶片组织提取物中的抗氧化能力提供了一种通用的诊断工具。