Lee Sangyeop, Choi Junghyun, Chen Lingxin, Park Byungchoon, Kyong Jin Burm, Seong Gi Hun, Choo Jaebum, Lee Yeonjung, Shin Kyung-Hoon, Lee Eun Kyu, Joo Sang-Woo, Lee Kyeong-Hee
Department of Applied Chemistry, Hanyang University, Ansan 426-791, South Korea.
Anal Chim Acta. 2007 May 8;590(2):139-44. doi: 10.1016/j.aca.2007.03.049. Epub 2007 Mar 28.
A rapid and highly sensitive trace analysis technique for determining malachite green (MG) in a polydimethylsiloxane (PDMS) microfluidic sensor was investigated using surface-enhanced Raman spectroscopy (SERS). A zigzag-shaped PDMS microfluidic channel was fabricated for efficient mixing between MG analytes and aggregated silver colloids. Under the optimal condition of flow velocity, MG molecules were effectively adsorbed onto silver nanoparticles while flowing along the upper and lower zigzag-shaped PDMS channel. A quantitative analysis of MG was performed based on the measured peak height at 1615 cm(-1) in its SERS spectrum. The limit of detection, using the SERS microfluidic sensor, was found to be below the 1-2 ppb level and this low detection limit is comparable to the result of the LC-Mass detection method. In the present study, we introduce a new conceptual detection technology, using a SERS microfluidic sensor, for the highly sensitive trace analysis of MG in water.
利用表面增强拉曼光谱（SERS）研究了一种用于在聚二甲基硅氧烷（PDMS）微流体传感器中测定孔雀石绿（MG）的快速且高灵敏度的痕量分析技术。制作了一个之字形的PDMS微流体通道，以实现MG分析物与聚集的银胶体之间的有效混合。在最佳流速条件下，MG分子在沿着上下之字形PDMS通道流动时有效地吸附到银纳米颗粒上。基于在其SERS光谱中1615 cm(-1)处测得的峰高对MG进行了定量分析。使用SERS微流体传感器的检测限被发现低于1 - 2 ppb水平，并且这个低检测限与液相色谱 - 质谱检测方法的结果相当。在本研究中，我们引入了一种使用SERS微流体传感器的新概念检测技术，用于水中MG的高灵敏度痕量分析。
