Strehle Katrin R, Cialla Dana, Rösch Petra, Henkel Thomas, Köhler Michael, Popp Jürgen
Department for Physical Chemistry, Friedrich-Schiller-University Jena, Helmholtzweg 4, D-07743 Jena, Germany.
Anal Chem. 2007 Feb 15;79(4):1542-7. doi: 10.1021/ac0615246.
The application of a liquid/liquid microsegmented flow for serial high-throughput microanalytical systems shows promising prospects for applications in clinical chemistry, pharmaceutical research, process diagnostics, and analytical chemistry. Microscopy and microspectral analytics offer powerful approaches for the analytical readout of droplet based assays. Within the generated segments, individuality and integrity are retained during the complete diagnostic process making the approach favored for analysis of individual microscaled objects like cells and microorganisms embedded in droplets. Here we report on the online application of surface-enhanced micro-Raman spectroscopy for the detection and quantization of analytes in a liquid/liquid segmented microfluidic system. Data acquisition was performed in microsegments down to a volume of 180 nl. With this approach, we overcome the well-known problem of adhesion of colloid/analyte conjugates to the optical windows of detection cuvettes, which causes the so-called "memory effect". The combination of the segmented microfluidic system with the highly sensitive SERS technique reaches in a reproducible quantification of analytes with the SERS technique.
液/液微分段流在串行高通量微分析系统中的应用在临床化学、药物研究、过程诊断和分析化学领域展现出了广阔的应用前景。显微镜和微光谱分析为基于液滴的分析提供了强大的分析读出方法。在生成的段内，个体性和完整性在整个诊断过程中得以保留，这使得该方法有利于分析嵌入液滴中的单个微观物体，如细胞和微生物。在此，我们报道了表面增强显微拉曼光谱在液/液分段微流控系统中对分析物进行检测和定量的在线应用。数据采集在低至180 nl体积的微段中进行。通过这种方法，我们克服了胶体/分析物共轭物粘附在检测比色皿光学窗口上这一众所周知的问题，该问题会导致所谓的“记忆效应”。分段微流控系统与高灵敏度表面增强拉曼光谱技术相结合，实现了用表面增强拉曼光谱技术对分析物进行可重复的定量分析。
