Ferreira T Alexandra, Guevara-Lara Alfredo, Paez-Hernandez M Elena, Mondragon Alicia C, Rodriguez Jose A
Area Academica de Quimica, Universidad Autonoma del Estado de Hidalgo Mineral de la Reforma Hidalgo Mexico
Laboratorio de Higiene, Inspeccion y Control de Alimentos, Departamento de Química Analitica, Nutricion y Bromatologia, Facultad de Veterinaria, Universidad de Santiago de Compostela 27002 Lugo Spain.
RSC Adv. 2021 Nov 2;11(56):35375-35382. doi: 10.1039/d1ra06301d. eCollection 2021 Oct 28.
In this work a simple and novel procedure for leucomalachite green determination based on micro flow injection analysis (μFIA) with amperometric detection is presented. The method involves the use of henequen modified fibers as microfluidic channels. The μFIA system proposed offers a simple, rapid, and low-cost alternative for the determination. Capillary and gravitational forces across the modified henequen fibers control the flow rate, eliminating the need for external pumps. This technique requires low reagent consumption and allows portability for measurements. The flow system is described, and the operational variables were studied and optimized using a Taguchi parameters design to increase analytical sensitivity. Under optimal conditions a limit of detection of 1.16 μg kg was achieved with adequate repeatability and reproducibility (expressed as %RSD <5.0%, = 3, = 9 respectively) in all cases. The effect of interfering species and the accuracy of the method were also investigated. The proposed methodology was validated and applied to determine LMG in tilapia muscle samples.
本文提出了一种基于微流控注射分析（μFIA）和安培检测的简单新颖的孔雀石绿测定方法。该方法采用剑麻改性纤维作为微流控通道。所提出的μFIA系统为测定提供了一种简单、快速且低成本的替代方案。改性剑麻纤维上的毛细管力和重力控制流速，无需外部泵。该技术试剂消耗低，便于携带进行测量。描述了流动系统，并使用田口参数设计研究和优化了操作变量以提高分析灵敏度。在最佳条件下，检测限达到1.16μg/kg，在所有情况下均具有足够的重复性和再现性（分别表示为%RSD<5.0%，n=3，N=9）。还研究了干扰物质的影响和方法的准确性。所提出的方法经过验证，并应用于罗非鱼肌肉样品中孔雀石绿的测定。
