Dai Li-Cheng, Wang Xiang, Yao Xing, Lu Yong-Liang, Ping Jin-Liang, He Jian-Fang
Huzhou Key Laboratory of Molecular Medicine, Huzhou Central Hospital, Huzhou 313000, Zhejiang Province, China.
World J Gastroenterol. 2007 Feb 28;13(8):1208-13. doi: 10.3748/wjg.v13.i8.1208.
To evaluate the effect of antisense oligonucleotide targeting midkine (MK-AS) on angiogenesis in chick chorioallantoic membrane (CAM) and in situ human hepatocellular carcinoma (HCC).
An in situ human hepatocellular carcinoma (HCC) model and CAM assay were used in this experiment. The effect of MK-AS on angiogenesis was evaluated by cell proliferation assay and hematoxylin-eosin (HE) staining.
MK-AS significantly inhibited human umbilical vein endothelial cells (HUVEC) and in situ human HCC growth. At the same time, MK-AS suppressed the angiogenesis both in human hepatocellular carcinoma cell line (HEPG2)-induced CAM and in situ human HCC tissues.
MK-AS is an effective antiangiogenesis agent in vivo.
评估靶向中期因子的反义寡核苷酸(MK-AS)对鸡胚绒毛尿囊膜(CAM)血管生成及人原位肝细胞癌(HCC)的影响。
本实验采用人原位肝细胞癌模型及CAM试验。通过细胞增殖试验和苏木精-伊红(HE)染色评估MK-AS对血管生成的影响。
MK-AS显著抑制人脐静脉内皮细胞(HUVEC)及人原位肝癌生长。同时,MK-AS抑制人肝癌细胞系(HEPG2)诱导的CAM及人原位肝癌组织中的血管生成。
MK-AS在体内是一种有效的抗血管生成剂。