Blanchette Craig D, Lin Wan-Chen, Orme Christine A, Ratto Timothy V, Longo Marjorie L
Biophysics Graduate Group, College of Biological Sciences, and Department of Chemical Engineering and Materials Science, University of California, Davis, California, 95616, USA.
Langmuir. 2007 May 22;23(11):5875-7. doi: 10.1021/la7004584. Epub 2007 Apr 24.
This work presents a novel method for experimentally quantifying interfacial line tension, which can be readily applied to study a wide variety of different lipid mixtures exhibiting phase coexistence. The method combines AFM imaging of lipid domain nucleation with classical nucleation theories. The results, using symmetric and asymmetric domains, permit the prediction of key physical parameters (critical nuclei size and nucleation rate) in multicomponent bilayer systems with implications toward understanding the dynamic nature of submicrometer domains (i.e., lipid rafts) in cell membranes.
这项工作提出了一种通过实验定量界面线张力的新方法，该方法可轻松应用于研究表现出相共存的各种不同脂质混合物。该方法将脂质域成核的原子力显微镜成像与经典成核理论相结合。使用对称和不对称域的结果，可以预测多组分双层系统中的关键物理参数（临界核尺寸和成核速率），这对于理解细胞膜中亚微米域（即脂筏）的动态性质具有重要意义。
