Nishino N, Hattori H, Kishida Y
Department of Animal Science, Faculty of Agriculture, Okayama University, Okayama, Japan.
Lett Appl Microbiol. 2007 May;44(5):538-43. doi: 10.1111/j.1472-765X.2006.02105.x.
To evaluate the fermentation characteristics and the effects of Lactobacillus buchneri inoculation in ensiling whole crop rice.
Laboratory-scale silages were prepared from whole crop rice harvested at yellow-ripe stage. The crop was ensiled for 2 months with and without inoculation of L. buchneri at 10(4), 10(5) and 10(6) CFU g(-1). The effect of prolonged ensiling was also studied by using the same crop; the silos were opened at 1, 3, 6 and 12 months, while the inoculation was made at 10(5) CFU g(-1). Enhanced alcoholic fermentation was found in untreated silage; the sum of ethanol and 2,3-butanediol were seven times higher at 2 months than those of lactic and volatile fatty acids, while the differences were diminished at 12 months owing to the reduction of ethanol in the late ensiling period. Inoculation of L. buchneri inhibited the alcohols; however, ethanol yet prevailed over the fermentation until 6 months, after which acetic acid became the main product in the inoculated silage. Regardless of inoculation and ensiling period, yeasts were not found in whole crop rice silage.
Substantial amounts of ethanol and 2,3-butanediol would be produced in silage prepared from whole crop rice. The alcoholic fermentation can be suppressed when inoculated with L. buchneri.
Inoculation of L. buchneri could be an option to prevent ethanol fermentation in silage.
评估布氏乳杆菌接种对全株水稻青贮发酵特性的影响。
以黄熟期收获的全株水稻制备实验室规模的青贮饲料。将作物在接种和未接种布氏乳杆菌（接种量分别为10⁴、10⁵和10⁶ CFU g⁻¹）的情况下青贮2个月。还使用相同作物研究了延长青贮时间的影响；青贮窖在1、3、6和12个月时打开，接种量为10⁵ CFU g⁻¹。在未处理的青贮饲料中发现酒精发酵增强；在2个月时，乙醇和2,3 - 丁二醇的总量比乳酸和挥发性脂肪酸高7倍，而在12个月时，由于后期青贮过程中乙醇减少，差异减小。接种布氏乳杆菌抑制了醇类物质；然而，直到6个月乙醇在发酵中仍占主导地位，之后接种的青贮饲料中乙酸成为主要产物。无论接种与否和青贮时间长短，全株水稻青贮饲料中均未发现酵母。
由全株水稻制备的青贮饲料中会产生大量乙醇和2,3 - 丁二醇。接种布氏乳杆菌可抑制酒精发酵。
接种布氏乳杆菌可能是防止青贮饲料中乙醇发酵的一种选择。
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