Falch Eva, Størseth Trond Røvik, Aursand Marit
SINTEF Fisheries and Aquaculture, Trondheim, Norway; The Norwegian University of Science and Technology, Department of Biotechnology, Trondheim, Norway.
Chem Phys Lipids. 2007 May;147(1):46-57. doi: 10.1016/j.chemphyslip.2007.03.006. Epub 2007 Mar 21.
High resolution NMR was applied to study biochemical changes of lipids in cod (Gadus morhua) gonads during 7 days storage at 4 degrees C. Changes were observed in the (13)C and (1)H resonances of cholesterol which were due to esterification of fatty acids at the hydroxyl position in roe and milt. Furthermore, the (13)C NMR spectra showed that the lipolytic changes in milt and roe where different. New resonances appeared during storage, due to formation of specific free fatty acids, with the corresponding changes in resonances of the esterified carbonyls and glycerols. The highly unsaturated n-3 fatty acids were hydrolysed from the sn-1 and sn-2 position of both phosphatidylcholine and phosphatidylethanolamine in milt. The lipolytical changes in roe were less prominent compared to the changes in milt, however significant levels of sn-1-lysophospholipids was detected both in roe and milt. The current data demonstrate that high resolution NMR may be a suitable method to non-destructively study hydrolysis and esterification reactions occurring in heterogeneous marine lipids in a one step procedure.
应用高分辨率核磁共振技术研究了鳕鱼（Gadus morhua）性腺在4℃下储存7天期间脂质的生化变化。观察到胆固醇的碳-13（¹³C）和氢-1（¹H）共振发生了变化，这是由于鱼卵和精液中脂肪酸在羟基位置发生了酯化反应。此外，碳-13核磁共振光谱显示，精液和鱼卵中的脂解变化有所不同。储存期间出现了新的共振峰，这是由于特定游离脂肪酸的形成，同时酯化羰基和甘油的共振峰也发生了相应变化。高度不饱和的n-3脂肪酸从精液中磷脂酰胆碱和磷脂酰乙醇胺的sn-1和sn-2位置水解下来。与精液中的变化相比，鱼卵中的脂解变化不太明显，不过在鱼卵和精液中均检测到了显著水平的sn-1-溶血磷脂。目前的数据表明，高分辨率核磁共振技术可能是一种合适的方法，可通过一步程序对异质海洋脂质中发生的水解和酯化反应进行无损研究。
