Sridhar M G, Setia Sajita, John Mathew, Bhat Vishnu, Nandeesha H, Sathiyapriya V
Department of Biochemistry, Jawaharlal Institute of Postgraduate Medical Education and Research, Pondicherry 605 006, India.
Clin Biochem. 2007 Jun;40(9-10):688-91. doi: 10.1016/j.clinbiochem.2007.01.023. Epub 2007 Mar 3.
To assess if oxidative injury in intrauterine growth retarded and healthy newborns is affected by the mode of delivery and whether Apgar score as a marker of neonatal survival is dependent on lipid and protein oxidative injury assessed by measuring malondialdehyde and protein carbonylation.
30 IUGR newborns, 15 born by normal vaginal delivery and 15 born by elective caesarean section, and 40 healthy control infants, 30 born by normal vaginal delivery and 10 born by elective caesarean section, were studied at birth. Cord blood was collected for determination of malondialdehyde and protein carbonylation.
IUGR newborns had significantly elevated MDA and protein carbonylation than the control group. Apgar scores, both at 1 and 5 min, were significantly lower in the IUGR group. These were assessed by independent sample t test. Using one-way ANOVA we found that MDA and protein carbonyls levels were not statistically different in healthy control group and in IUGR subgroup born by caesarean section however they were significantly different in other groups. Linear regression analysis revealed that Apgar scores both at 1 min and 5 min were dependent on MDA in IUGR newborns. Apgar score was however not dependent on protein carbonylation in the same group.
Apgar score in IUGR newborns is dependent on the extent of oxidative injury and elective caesarean section minimises the same.
评估宫内生长受限（IUGR）新生儿和健康新生儿的氧化损伤是否受分娩方式影响，以及作为新生儿生存标志物的阿氏评分是否依赖于通过测量丙二醛和蛋白质羰基化评估的脂质和蛋白质氧化损伤。
研究了30例IUGR新生儿（15例经正常阴道分娩，15例经择期剖宫产）和40例健康对照婴儿（30例经正常阴道分娩，10例经择期剖宫产）出生时的情况。采集脐血以测定丙二醛和蛋白质羰基化。
IUGR新生儿的丙二醛和蛋白质羰基化水平显著高于对照组。IUGR组1分钟和5分钟时的阿氏评分均显著较低。这些通过独立样本t检验进行评估。使用单因素方差分析，我们发现健康对照组和剖宫产出生的IUGR亚组中丙二醛和蛋白质羰基水平无统计学差异，但在其他组中差异显著。线性回归分析显示，IUGR新生儿1分钟和5分钟时的阿氏评分均依赖于丙二醛。然而，同一组中阿氏评分不依赖于蛋白质羰基化。
IUGR新生儿的阿氏评分依赖于氧化损伤程度，择期剖宫产可将其降至最低。
