Gutsmann Thomas, Schromm Andra B, Brandenburg Klaus
Forschungszentrum Borstel, Leibniz-Zentrum für Medizin und Biowissenschaften, Parkallee 10, D-23845 Borstel, Germany.
Int J Med Microbiol. 2007 Sep;297(5):341-52. doi: 10.1016/j.ijmm.2007.03.004. Epub 2007 Apr 23.
It is generally accepted that the interaction of bacterial pathogenicity factors such as endotoxin (lipopolysaccharide, LPS) with molecules and cells of the human immune system, which eventually may lead to pathophysiological effects like septic shock, is exerted by isolated molecules after their release from the bacteria. Therefore, the study of the direct, physical interaction of LPS with target structures by applying biophysical means is of high interest. The questions which arise in this context concern the biologically active unit of LPS (monomer, multimer, aggregate), the molecular conformation of the single molecules, the type of aggregation of LPS polymers, the strength of their binding to serum and membrane proteins and/or unspecific binding to membrane phospholipids. Here, recent progress is reviewed which has increased our understanding of the processes preceding LPS-induced immune cell activation.
人们普遍认为，诸如内毒素（脂多糖，LPS）等细菌致病因子与人类免疫系统的分子和细胞之间的相互作用最终可能导致诸如败血症休克等病理生理效应，这种相互作用是由这些分子从细菌中释放后单独发挥的。因此，应用生物物理方法研究LPS与靶结构的直接物理相互作用备受关注。在这种情况下出现的问题涉及LPS的生物活性单位（单体、多聚体、聚集体）、单个分子的分子构象、LPS聚合物的聚集类型、它们与血清和膜蛋白结合的强度以及/或者与膜磷脂的非特异性结合。在此，我们综述了最近的进展，这些进展增进了我们对LPS诱导免疫细胞激活之前过程的理解。
