Schweda Elke K H, Richards James C, Hood Derek W, Moxon E Richard
Clinical Research Centre, Karolinska Institutet and University College of South Stockholm, NOVUM, S-141 86 Huddinge, Sweden.
Int J Med Microbiol. 2007 Sep;297(5):297-306. doi: 10.1016/j.ijmm.2007.03.007. Epub 2007 Apr 23.
Lipopolysaccharide (LPS) is a major virulence determinant of the human bacterial pathogen Haemophilus influenzae. A characteristic feature of H. influenzae LPS is the extensive intra- and inter-strain heterogeneity of glycoform structure which is key to the role of the molecule in both commensal and disease-causing behaviour of the bacterium. Through the combination of genetics and detailed structural analyses, H. influenzae is an exemplar Gram-negative bacterium for which now the most extensive and detailed LPS structural data and functional correlates are available. LPS from H. influenzae consists of a conserved glucose-substituted triheptosyl inner-core moiety l-alpha-d-Hepp-(1-->2)-[PEtn-->6]-l-alpha-d-Hepp-(1-->3)-[beta-d-Glcp-(1-->4)]-l-alpha-d-Hepp linked to lipid A via Kdo 4-phosphate. The inner-core unit provides the template for attachment of oligosaccharide- and non-carbohydrate substituents. Here, the structure, genetics and expression of LPS glycoforms in the outer core are reviewed as well as their implication on virulence.
脂多糖（LPS）是人类细菌病原体流感嗜血杆菌的主要毒力决定因素。流感嗜血杆菌LPS的一个特征是糖型结构在菌株内和菌株间存在广泛的异质性，这是该分子在细菌共生和致病行为中发挥作用的关键。通过遗传学与详细结构分析相结合，流感嗜血杆菌是一种典型的革兰氏阴性菌，目前已有关于其最广泛和详细的LPS结构数据及功能相关性。流感嗜血杆菌的LPS由一个保守的葡萄糖取代的三庚糖基内核部分l-α-d-Hepp-(1→2)-[PEtn→6]-l-α-d-Hepp-(1→3)-[β-d-Glcp-(1→4)]-l-α-d-Hepp通过Kdo 4-磷酸与脂质A相连组成。内核单元为寡糖和非碳水化合物取代基的连接提供了模板。在此，对外核中LPS糖型的结构、遗传学和表达及其对毒力的影响进行综述。
