Takehara M
Arch Virol. 1975;49(4):297-306. doi: 10.1007/BF01318238.
Cytopathological effects by vesicular stomatitis virus (VSV) infection were studied in several cell lines. Marked polykaryocyte formation was observed in monolayers of certain strains of BHK-21 cells infected with VSV. The BHK-21-KB cells were found to be the most susceptible to virus-induced cell fusion. This type of cell fusion was related to intracellular growth of the viruses, and strong cytolytic changes were found to occur following the development of large multinucleated giant cells. The cell-fusing activity was associated with the infectivity of VSV and was neutralized by anti-VSV immune serum. The viruses irradiated for 20 minutes or heated at 60 degrees C for 10 minutes lost completely both infectivity and cell-fusing activity. These experimental results indicate that virus replication was responsible for fusion of BHK cells.
在几种细胞系中研究了水泡性口炎病毒（VSV）感染的细胞病理学效应。在用VSV感染的某些BHK - 21细胞株的单层细胞中观察到明显的多核细胞形成。发现BHK - 21 - KB细胞对病毒诱导的细胞融合最敏感。这种类型的细胞融合与病毒的细胞内生长有关，并且在大型多核巨细胞形成后发现发生了强烈的细胞溶解变化。细胞融合活性与VSV的感染性相关，并被抗VSV免疫血清中和。照射20分钟或在60摄氏度加热10分钟的病毒完全丧失了感染性和细胞融合活性。这些实验结果表明病毒复制是BHK细胞融合的原因。
