Murakami Aya, Ishikawa Takashi, Takechi Kenshi, Ago Jun, Matsumoto Naotaka, Kamei Chiaki
Department of Medicinal Pharmacology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Japan.
J Pharmacol Sci. 2007 May;104(1):56-60. doi: 10.1254/jphs.fp0061487. Epub 2007 Apr 24.
The changes of electromyographic activity (EMG seizure) induced by maximal electroshock were studied in comparison with those of behavioral seizures in mice. In addition, the effects of certain antiepileptics on behavioral seizures and EMG seizure induced by maximal electroshock were also studied. High amplitude with high frequency EMG seizure was observed in parallel with the appearance of tonic extensor (TE) seizure and an intimate relationship was observed between the two parameters. On the other hand, to investigate the intensity of TE seizure, the product of the amplitude and the duration in EMG seizure was calculated, and the effects of antiepileptics on the magnitude of EMG seizure were investigated. As a result, a significant difference was observed at the doses of antiepileptics that showed no significant effects on the durations of TE and EMG seizures; that is, phenytoin, phenobarbital, topiramate, and carbamazepine showed significant effects on the magnitude of EMG seizure at doses of 5, 2, 10, and 5 mg/kg, respectively. From these findings, it may be concluded that this index, that is, the magnitude of EMG seizure induced by maximal electroshock, is a more reliable and highly sensitive method for the assessment of the potential activity of antiepileptics.
将小鼠最大电休克诱发的肌电图活动变化（肌电图癫痫发作）与行为性癫痫发作变化进行了比较研究。此外，还研究了某些抗癫痫药物对最大电休克诱发的行为性癫痫发作和肌电图癫痫发作的影响。观察到高幅度高频肌电图癫痫发作与强直性伸展（TE）癫痫发作同时出现，且两个参数之间存在密切关系。另一方面，为了研究TE癫痫发作的强度，计算了肌电图癫痫发作中幅度与持续时间的乘积，并研究了抗癫痫药物对肌电图癫痫发作幅度的影响。结果发现，在对TE和肌电图癫痫发作持续时间无显著影响的抗癫痫药物剂量下存在显著差异；即苯妥英、苯巴比妥、托吡酯和卡马西平分别在5、2、10和5mg/kg剂量下对肌电图癫痫发作幅度有显著影响。从这些发现可以得出结论，这个指标，即最大电休克诱发的肌电图癫痫发作幅度，是评估抗癫痫药物潜在活性的更可靠、更敏感的方法。
