Hannink Gerjon, Schreurs B Willem, Buma Pieter
Orthopaedic Research Lab, Radboud University Nijmegen Medical Centre, P.O. Box 9101, Nijmegen, HB 6500, the Netherlands.
Acta Orthop. 2007 Feb;78(1):31-8. doi: 10.1080/17453670610013394.
Gamma irradiation has been widely used for sterilization of bone allografts. However, gamma irradiation alters proteins. This is favorable when it reduces immunogenicity, but is undesirable when osteoinductive proteins are damaged. Although the effect of gamma irradiation on BMPs has been studied, the effect of irradiation on the process of incorporation of morselized bone chips remains unclear. We studied the effects of sterilization by gamma irradiation on the incorporation of impacted morselized allografts.
Bone chambers with impacted allografts, rinsed impacted allografts, allografts that were rinsed and subsequently irradiated, and an empty control were implanted in proximal medial tibiae of goats. Incorporation was evaluated using histology and histomorphometry.
Histology revealed evidence of bone graft incorporation, which proceeded in a similar way in unprocessed, rinsed, and both rinsed and irradiated bone grafts. After 12 weeks, no difference in bone and tissue ingrowth was found between the unprocessed, the rinsed, and the rinsed and subsequently irradiated allografts. The amount of unresorbed graft remnant was highest in the unprocessed bone grafts.
We conclude that sterilization with gamma irradiation does not influence the incorporation of impacted rinsed bone allografts.
伽马射线辐照已广泛用于同种异体骨的灭菌。然而，伽马射线辐照会改变蛋白质。当它降低免疫原性时这是有利的，但当骨诱导蛋白受损时则是不利的。尽管已经研究了伽马射线辐照对骨形态发生蛋白（BMPs）的影响，但辐照对碎骨片植入过程的影响仍不清楚。我们研究了伽马射线辐照灭菌对嵌入碎骨同种异体骨植入的影响。
将装有嵌入同种异体骨的骨腔、冲洗后的嵌入同种异体骨、冲洗后再辐照的同种异体骨以及一个空对照植入山羊胫骨近端内侧。使用组织学和组织形态计量学评估植入情况。
组织学显示有骨移植植入的证据，未处理的、冲洗后的以及冲洗后再辐照的骨移植以相似的方式进行植入。12周后，未处理的、冲洗后的以及冲洗后再辐照的同种异体骨在骨和组织长入方面未发现差异。未吸收的移植残余物量在未处理的骨移植中最高。
我们得出结论，伽马射线辐照灭菌不影响冲洗后嵌入骨同种异体骨的植入。
