Shakeri Shahryar, Kermanshahi Roha Kasra, Moghaddam Madjid Momeni, Emtiazi Giti
Department of Biology, Faculty of Sciences, Isfahan University, Isfahan, Iran.
Biofouling. 2007;23(1-2):79-86. doi: 10.1080/08927010701190011.
Biofilm formation on surfaces has serious economic and environmental implications. Growth of biofilm within a water distribution system can lead to problems such as biocorrosion and biofouling accumulation. To prevent and control these occurrences, it is necessary to use suitable biocides to remove the biofilm and kill biofilm cells. In this study, the genera Actinobacillus, Branhamella, Bacillus, Micrococcus and Acinetobacter were isolated from biofilms formed on brass coupons exposed to a cooling water system. It was shown by the microtiter plate test that a mixed culture of the isolates and a single culture of Acinetobacter sp(2) produced high levels of biofilm formation. A microwell plate technique was applied for assessment of the ability of various biocides to remove and kill mixed-culture biofilm cells and Acinetobacter sp(2), the latter as a single-species biofilm with a high rate of biofilm production. The results showed that the mixed-culture biofilm cells had more resistance to removal and killing by some biocides, such as hydrogen peroxide and sulfathiazole, than the single-species biofilm cells (Acinetobacter sp(2)). Oxidising biocides, such as sodium hypochlorite and hydrogen peroxide, demonstrated a higher potential for biofilm removal and killing compared with non-oxidising biocides (sulfathiazole and glutaraldehyde).
表面生物膜的形成具有严重的经济和环境影响。配水系统中生物膜的生长会导致生物腐蚀和生物污垢积累等问题。为了预防和控制这些情况，有必要使用合适的杀菌剂来去除生物膜并杀死生物膜细胞。在本研究中，放线杆菌属、布兰汉氏菌属、芽孢杆菌属、微球菌属和不动杆菌属是从暴露于冷却水系统的黄铜试片上形成的生物膜中分离出来的。微量滴定板试验表明，分离菌株的混合培养物和不动杆菌sp(2)的单一培养物产生了高水平的生物膜形成。采用微孔板技术评估了各种杀菌剂去除和杀死混合培养生物膜细胞以及不动杆菌sp(2)（后者作为生物膜产生率高的单物种生物膜）的能力。结果表明，与单物种生物膜细胞（不动杆菌sp(2)）相比，混合培养生物膜细胞对某些杀菌剂（如过氧化氢和磺胺噻唑）的去除和杀灭具有更高的抗性。与非氧化性杀菌剂（磺胺噻唑和戊二醛）相比，氧化性杀菌剂（如次氯酸钠和过氧化氢）在生物膜去除和杀灭方面表现出更高的潜力。
