Burgess Jefferey L, Fleming Joy E, Mulenga Emmanuel M, Josyula Arun, Hysong Tracy A, Joggerst Philip J, Kurzius-Spencer Margaret, Miller Hugh B
University of Arizona, Tucson, Arizona 85724-5210, USA.
Clin Toxicol (Phila). 2007;45(3):255-60. doi: 10.1080/15563650601072142.
Although exposure to diesel exhaust has been linked with adverse health effects, little is known about the acute effects of exposure in the underground workplace.
Cross-shift spirometry and sputum induction were completed on twelve subjects associated with comminuted rock removal (mucking) operations in an underground copper mine using diesel powered and pneumatic equipment on separate days, and sputum collected on a baseline non-exposure day as well.
For diesel operations, elemental carbon exposure averaged 538 +/- 512 microg/m(3) during the 1-2 hour operations. Sputum interleukin-10 decreased with diesel exhaust using one ELISA assay (3.69 v. 2.32 pg/ml, p = 0.015), but increased when measured with a different ELISA kit (0.18 v. 0.59 pg/ml, p = 0.019), consistent with an overall decline in IL-10 protein concentration but an increase in the biologically active form. Sputum interleukin-6 decreased with exposure to diesel exhaust, although this change lost statistical significance when restricted to non-smokers. There were no significant changes in spirometry, interleukins 1beta, 4, and 8, tumor necrosis factor alpha or 8-hydroxy-2'-deoxyguanosine.
High levels of diesel exhaust can result in rapid changes in sputum IL-10, suggesting possible protein modification.
尽管接触柴油机尾气与不良健康影响有关，但对于地下工作场所接触柴油机尾气的急性影响知之甚少。
对12名与地下铜矿中使用柴油动力和气动设备进行破碎岩石清除（出渣）作业相关的受试者，在不同日期分别进行了跨班次肺活量测定和痰液诱导，并在非接触基线日也采集了痰液。
对于柴油作业，在1 - 2小时的作业过程中，元素碳暴露平均为538±512微克/立方米。使用一种酶联免疫吸附测定法（ELISA）时，痰液白细胞介素 - 10随柴油机尾气暴露而降低（3.69对2.32皮克/毫升，p = 0.015），但使用不同的ELISA试剂盒测量时则升高（0.18对0.59皮克/毫升，p = 0.019），这与白细胞介素 - 10蛋白浓度总体下降但生物活性形式增加一致。痰液白细胞介素 - 6随柴油机尾气暴露而降低，尽管这种变化在仅限于非吸烟者时失去统计学意义。肺活量测定、白细胞介素1β、4和8、肿瘤坏死因子α或8 - 羟基 - 2'-脱氧鸟苷没有显著变化。
高浓度的柴油机尾气可导致痰液白细胞介素 - 10迅速变化，提示可能存在蛋白质修饰。
