使用极低频皮肤激光多普勒血流光谱对人体微血管一氧化氮功能进行无创测量。
Noninvasive measure of microvascular nitric oxide function in humans using very low-frequency cutaneous laser Doppler flow spectra.
作者信息
Stewart Julian M, Taneja Indu, Goligorsky Michael S, Medow Marvin S
机构信息
New York Medical College, Valhalla, New York, USA.
出版信息
Microcirculation. 2007 Apr-May;14(3):169-80. doi: 10.1080/10739680601139179.
OBJECTIVE
While higher frequency oscillations (0.021-0.6 Hz) in cutaneous blood flow measured by laser Doppler flowmetry (LDF) relate to oscillations in blood pressure and sympathetic nerve activity, very low-frequency oscillations (VLF, 0.0095-0.021 Hz) do not. The authors investigated whether VLF LDF power is nitric oxide (NO) specific.
METHODS
LDF combined with intradermal microdialysis was used in the calves of 22 healthy volunteers aged 19-27 years. LDF power spectral analysis was performed by windowed fast Fourier transform. The authors tested whether the NO synthesis inhibitor nitro-l-arginine (NLA) produced selective decreases in VLF power before and after stimulation with acetylcholine.
RESULTS
NLA alone did not alter total power but selectively reduced VLF power by approximately 50%. LDF and spectral power increased markedly across all spectra with acetylcholine. This increase was blunted by NLA, which selectively reduced VLF power by approximately 50%.
CONCLUSIONS
The data suggest that VLF oscillations in the laser Doppler signal are NO dependent, increase with cholinergic stimulation, and have potential as a noninvasive marker for NO-dependent microvascular reactivity.
目的
虽然通过激光多普勒血流仪（LDF）测量的皮肤血流中的高频振荡（0.021 - 0.6赫兹）与血压和交感神经活动的振荡有关，但极低频振荡（VLF，0.0095 - 0.021赫兹）并非如此。作者研究了VLF LDF功率是否具有一氧化氮（NO）特异性。
方法
对22名年龄在19 - 27岁的健康志愿者的小腿使用LDF结合皮内微透析。通过加窗快速傅里叶变换进行LDF功率谱分析。作者测试了在乙酰胆碱刺激前后，NO合成抑制剂硝基 - L - 精氨酸（NLA）是否会使VLF功率选择性降低。
结果
单独使用NLA不会改变总功率，但会选择性地将VLF功率降低约50%。使用乙酰胆碱后，LDF和频谱功率在所有频谱上均显著增加。这种增加被NLA减弱，NLA选择性地将VLF功率降低约50%。
结论
数据表明，激光多普勒信号中的VLF振荡依赖于NO，随胆碱能刺激增加，并且有潜力作为NO依赖性微血管反应性的非侵入性标志物。
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