Reyes R, Valladares F, Díaz-Flores L, Feria L, Alonso R, Tramu G, Bello A R
Section of Cell Biology, School of Biology, University of La Laguna, La Laguna, Spain.
Histol Histopathol. 2007 Jul;22(7):709-17. doi: 10.14670/HH-22.709.
This study assesses the action of hypercortisolism on the hormone and peptide periadenoma region of removed ACTH-producing microadenoma. Our findings show that cortisol excess affects both ACTH and GH production, with no immunoreaction for these hormones. The remaining pituitary hormones (TSH, FSH and PRL) and POMC-derived peptides (betaEnd, alphaMSH and betaMSH) were not modified. Likewise, we observed pituitary immunoreactive cells for Neurotensin (NT), Intestinal vasoactive peptide (VIP), Substance P (SP) and Angiotensin-II (Ang-II). The colocalization demonstrated that NT was expressed in thyrotrope and gonadotrope cells, VIP in gonadotrope cells and SP in corticotrope cells. The results about Ang-II were inconclusive. On the other hand, immunoreaction for the NPY and Gal peptides were not present. In the adenomatous cells, the peptide NT is present in ACTH cells as well as SP. These results suggest a peptide regulation of pituitary cells in the pathological state that can differ between normal and tumoural cells of the same pituitary.
本研究评估了高皮质醇血症对切除的促肾上腺皮质激素（ACTH）分泌性微腺瘤周围腺区激素和肽的作用。我们的研究结果表明，皮质醇过量会影响ACTH和生长激素（GH）的分泌，且这些激素无免疫反应。其余垂体激素（促甲状腺激素（TSH）、促卵泡激素（FSH）和催乳素（PRL））以及源自阿黑皮素原（POMC）的肽（β内啡肽（betaEnd）、α促黑素（alphaMSH）和β促黑素（betaMSH））未发生改变。同样，我们观察到垂体中存在神经降压素（NT）、血管活性肠肽（VIP）、P物质（SP）和血管紧张素II（Ang-II）的免疫反应性细胞。共定位显示，NT在促甲状腺激素细胞和促性腺激素细胞中表达，VIP在促性腺激素细胞中表达，SP在促肾上腺皮质激素细胞中表达。关于Ang-II的结果尚无定论。另一方面，未观察到神经肽Y（NPY）和甘丙肽（Gal）肽的免疫反应。在腺瘤细胞中，肽NT以及SP存在于ACTH细胞中。这些结果表明，在病理状态下，垂体细胞存在肽调节，这在同一体内正常细胞和肿瘤细胞之间可能存在差异。
