Reyes R, Valladares F, Gutiérrez R, González M, Bello A R
Section of Cell Biology, School of Biology, University of La Laguna and FICIC, La Laguna, Tenerife, Spain.
J Anat. 2008 Jun;212(6):817-26. doi: 10.1111/j.1469-7580.2008.00906.x.
We have studied here the cellular distribution of several regulatory peptides in hormone-producing cells of the human pituitary during the fetal period. Immunohistochemistry was used to show the expression of several regulatory peptides, namely Angiotensin-II, Neurotensin and Galanin, at successive gestational stages and their co-localization with hormones in the human fetal adenohypophysis. Somatotrophs, gonadotrophs and thyrotrophs were differentiated earliest. At gestational week 9, Angiotensin-II immunoreactivity was co-localized only with growth hormone immunoreactivity in somatotrophs, one of the first hormone-producing cells to differentiate. This co-localization remained until week 37. Neurotensin immunoreactivity was present in gonadotrophs and thyrotrophs in week 23, after FSH and TSH hormone differentiation. Galanin immunoreactivity was present in all hormone-producing cell types except corticotrophs. The different pro-opiomelanocortin-derived peptides were detected at different stages of gestation and adrenocorticotrophic hormone immunoreaction was the last to be detected. Our results show an interesting relationship between regulatory peptides and hormones during human fetal development, which could imply that these peptides play a regulatory role in the development of pituitary function.
我们在此研究了胎儿期人垂体激素分泌细胞中几种调节肽的细胞分布情况。采用免疫组织化学方法来显示几种调节肽，即血管紧张素 - II、神经降压素和甘丙肽，在连续妊娠阶段的表达情况以及它们在人胎儿腺垂体中与激素的共定位。生长激素细胞、促性腺激素细胞和促甲状腺激素细胞最早分化。在妊娠第9周时，血管紧张素 - II免疫反应性仅在生长激素细胞中与生长激素免疫反应性共定位，生长激素细胞是最早分化的激素分泌细胞之一。这种共定位一直持续到第37周。在促卵泡激素和促甲状腺激素分化后的第23周，神经降压素免疫反应性出现在促性腺激素细胞和促甲状腺激素细胞中。甘丙肽免疫反应性存在于除促肾上腺皮质激素细胞外的所有激素分泌细胞类型中。不同的阿片 - 黑素 - 促皮质素原衍生肽在妊娠的不同阶段被检测到，促肾上腺皮质激素免疫反应是最后被检测到的。我们的结果显示了人胎儿发育过程中调节肽与激素之间存在有趣的关系，这可能意味着这些肽在垂体功能发育中发挥调节作用。
