Li You-Xin, Mao Jing-Yuan, Li Hui-Fen
Pharmacy College, Tianjin Medical University, Tianjin 300070, China.
Zhongguo Zhong Yao Za Zhi. 2007 Feb;32(4):285-8, 326.
Digoxin plays a part in healing of congestive heart failure in clinic. Its therapeutic dose is very approximate to toxic dose and even they overlap each other sometimes. There are many influencing factors on blood drug level of digoxin. Pharmacodynamics and pharmacokinetics varies with different individuality. It is indispensable to detecting blood drug level in order to treat disease and prevent intoxication. Integrating with the detecting-methods of blood drug level of digoxin home and broad, characteristic of many methods are summarized from sensitivity, linearity range, cross-reaction and precision. These methods include radio immunoassay, enzyme immunoassay, chemiluminescence immunoassay, fluorescence immunoassay and HPLC-MS-MS. These methods are popular for their specialized ascendancy. The cost of radio immunoassay is low. Enzyme immunoassay has good specificity. Sensitivity and stability of chemiluminescence immunoassay is very excellent. Fluorescence polarization immunoassay is sensitive and convenient. HPLC-MS-MS has high resolution and good specificity. One of the development tendencies is to combine two or more methods in detecting the blood drug level of digoxin which contribute to these methods integrated use.
地高辛在临床上对充血性心力衰竭的治疗有一定作用。其治疗剂量与中毒剂量非常接近，甚至有时二者相互重叠。影响地高辛血药浓度的因素众多。药物动力学和药效学因个体差异而有所不同。为了治疗疾病和预防中毒，检测血药浓度是必不可少的。结合国内外地高辛血药浓度的检测方法，从灵敏度、线性范围、交叉反应和精密度等方面总结了多种方法的特点。这些方法包括放射免疫分析法、酶免疫分析法、化学发光免疫分析法、荧光免疫分析法和高效液相色谱-串联质谱法。这些方法因其各自的专业优势而受到广泛应用。放射免疫分析法成本较低。酶免疫分析法具有良好的特异性。化学发光免疫分析法的灵敏度和稳定性非常出色。荧光偏振免疫分析法灵敏且便捷。高效液相色谱-串联质谱法具有高分辨率和良好的特异性。未来的发展趋势之一是将两种或更多种方法结合用于检测地高辛血药浓度，这有助于这些方法的综合应用。
