Jones S R, Woo P T
Department of Zoology, University of Guelph, Ontario, Canada.
Parasitology. 1991 Oct;103 Pt 2:237-43. doi: 10.1017/s0031182000059515.
Culture characteristics of two species of piscine trypanosomes were compared. Trypanosoma catostomi from Catostomus commersoni was isolated in hypo-osmotic blood agar media but not in hyper-osmotic media. However, T. phaleri from Amia calva was isolated in both media. Only T. catostomi was stimulated to divide by diluting infected blood with water. The maximum number of T. phaleri and percentage of trypomastigotes were not affected by NaCl concentration. The maximum number of T. catostomi was inversely related to osmotic pressure and the percentage of trypomastigotes was directly related to osmotic pressure. This was confirmed by using equi-osmotic concentrations of NaCl, NaBr, Na acetate, KCl, choline-Cl or sucrose. Supplementation with urea had no effect on the number or morphology of trypanosomes. The survival of T. catostomi following cryopreservation was enhanced by prior incubation in hyper-osmotic media. Differential culture characteristics of these species may reflect adaptation to different host-vector systems.
对两种鱼类锥虫的培养特性进行了比较。来自康氏美洲红点鲑的卡氏锥虫在低渗血琼脂培养基中可分离得到，但在高渗培养基中则不能。然而，来自雀鳝的法氏锥虫在两种培养基中均可分离得到。只有卡氏锥虫可通过用水稀释感染血液来刺激其分裂。法氏锥虫的最大数量和锥鞭毛体百分比不受氯化钠浓度的影响。卡氏锥虫的最大数量与渗透压呈负相关，而锥鞭毛体百分比与渗透压呈正相关。使用等渗浓度的氯化钠、溴化钠、醋酸钠、氯化钾、氯化胆碱或蔗糖证实了这一点。添加尿素对锥虫的数量或形态没有影响。在高渗培养基中预先培养可提高卡氏锥虫冷冻保存后的存活率。这些物种不同的培养特性可能反映了它们对不同宿主 - 媒介系统的适应性。
