Li S, Woo P T
Department of Zoology, University of Guelph, Ontario, Canada.
Parasitol Res. 1996;82(3):276-8. doi: 10.1007/s004360050112.
Cryptobia catostomi, a non-pathogenic haemoflagellate of white suckers (Catostomus commersoni) multiplied rapidly in modified TDL-15 medium supplemented with 10% fetal bovine serum and 1% heatinactivated white sucker serum (WSS) at 10 degrees C and 18 degrees C. The numbers of C. catostomi counted were significantly higher in cultures incubated at 10 degrees C than in those incubated at 18 degrees C beginning at 3 weeks post-incubation. The culture forms (from the eighth subculture at 9 months after isolation) were morphologically similar to blood forms and were infective to laboratory-raised white suckers. The parasite survived for about 4 weeks in the medium without WSS at 18 degrees C. The present study indicates that WSS supplement supports the in vitro multiplication of C. catostomi and that its multiplication is temperature-dependent.
隐鞭毛虫（Cryptobia catostomi）是白亚口鱼（Catostomus commersoni）的一种非致病性血鞭毛虫，在添加10%胎牛血清和1%热灭活白亚口鱼血清（WSS）的改良TDL - 15培养基中，于10摄氏度和18摄氏度时能快速繁殖。培养3周后，在10摄氏度下培养的隐鞭毛虫数量显著高于在18摄氏度下培养的数量。培养形式（分离9个月后的第八代传代培养）在形态上与血液中的形态相似，并且对实验室饲养的白亚口鱼具有感染性。该寄生虫在18摄氏度无WSS的培养基中存活约4周。本研究表明，添加WSS能支持隐鞭毛虫的体外增殖，且其增殖具有温度依赖性。
