Schwarzová K, Betáková T, Neméth J, Mizáková A
Institute of Virology, Slovak Academy of Sciences, Bratislava, Slovakia.
Folia Microbiol (Praha). 2006;51(6):653-8. doi: 10.1007/BF02931634.
We have screened 91 migratory birds representing 32 species during the autumn of 2003 for the presence of the zoonotic pathogens Borrelia and Chlamydophila. Using polymerase chain reaction (PCR), B. burgdorferi sensu stricto was detected in cloacal swabs and, in two causes, also in throat swabs in 8 individuals (8.7 %) representing 7 birds species; B. garinii and B. afzelii were not detected. C. psittaci was detected only in cloacal swabs; 6 birds (6.6 %) from four species were found to be positive. The PCR products were sequenced and the sequences were compared phylogenetically with the gene sequences of 14 Chlamydophila strains retrieved from nucleotide databases; although the sequenced DNA was only 110 bp long, all obtained sequences created a new cluster with sublines branching from a position close to the periphery of the genus. All tested samples appear distinct within the known species and were most similar to C. felis or C. abortis.
2003年秋季，我们对代表32个物种的91只候鸟进行了筛查，以检测人畜共患病原体伯氏疏螺旋体和鹦鹉热衣原体的存在。使用聚合酶链反应（PCR），在8只个体（8.7%）的泄殖腔拭子中检测到狭义伯氏疏螺旋体，在两例中，7种鸟类的咽喉拭子中也检测到该病原体；未检测到伽氏疏螺旋体和阿氏疏螺旋体。鹦鹉热衣原体仅在泄殖腔拭子中检测到；发现来自四个物种的6只鸟（6.6%）呈阳性。对PCR产物进行测序，并将序列与从核苷酸数据库中检索到的14株鹦鹉热衣原体菌株的基因序列进行系统发育比较；尽管测序的DNA只有110 bp长，但所有获得的序列都形成了一个新的簇，其子系从靠近该属边缘的位置分支出来。所有测试样本在已知物种中显得不同，并且与猫衣原体或流产衣原体最为相似。
