Huang Ke-Jing, Zhang Min, Xie Wan-Zhen, Zhang Hua-Shan, Feng Yu-Qi, Wang Hong
Department of Chemistry, Wuhan University, Wuhan 430072, PR China.
Anal Chim Acta. 2007 May 15;591(1):116-22. doi: 10.1016/j.aca.2007.03.052. Epub 2007 Mar 30.
Nitric oxide (NO) plays a very important role in human blood system. In this work, a novel approach has been developed for the quantitation of ultra-trace NO derivatized with 1,3,5,7-tetramethyl-8-(3',4'-diaminophenyl)-difluoroboradiaza-s-indacene (DAMBO) using a polymer monolith microextraction (PMME) with a poly(methacrylic acid-ethylene glycol dimethacrylate) (MAA-EGDMA) monolith in conjunction with high-performance liquid chromatography (HPLC). Both derivatization and PMME conditions have been optimized in detail. The detection limit of derivatized NO was 2x10(-12) mol L(-1) (signal to noise=3) and linear range was 9x10(-11)-4.5x10(-8) mol L(-1). The proposed DAMBO-based derivatization-PMME-HPLC-fluorescence detection method has been successfully applied for the determination of NO in 10 microL blood samples of healthy persons and patients suffering from ischemic cardio-cerebrovascular diseases with recoveries varying from 87.40 to 91.60%.
一氧化氮（NO）在人体血液系统中起着非常重要的作用。在本研究中，开发了一种新方法，用于定量分析用1,3,5,7-四甲基-8-（3',4'-二氨基苯基）-二氟硼二氮杂-s-茚（DAMBO）衍生化的超痕量NO，该方法采用聚（甲基丙烯酸-乙二醇二甲基丙烯酸酯）（MAA-EGDMA）整体柱的聚合物整体微萃取（PMME）结合高效液相色谱（HPLC）。衍生化和PMME条件均已进行了详细优化。衍生化NO的检测限为2×10⁻¹² mol L⁻¹（信噪比=3），线性范围为9×10⁻¹¹ - 4.5×10⁻⁸ mol L⁻¹。所提出的基于DAMBO的衍生化-PMME-HPLC-荧光检测方法已成功应用于健康人和缺血性心脑血管疾病患者10 μL血样中NO的测定，回收率在87.40%至91.60%之间。
