Huang Ke-Jing, Xie Wan-Zhen, Wang Hong, Zhang Hua-Shan
Department of Chemistry, Wuhan University, Wuhan, PR China.
Talanta. 2007 Aug 15;73(1):62-7. doi: 10.1016/j.talanta.2007.02.031. Epub 2007 Mar 1.
S-Nitrosothiols (RSNO) have been proved to be potent smooth muscle relaxants and inhibitors of platelet aggregation. It has been reported as the best candidate for the endogenous storage and transport of nitric oxide (NO) in vivo. Sensitive determination of RSNO in biosamples seems to be of great significance. In this work, a novel spectrofluorimetric method is proposed to determine RSNO. An excellent fluorescence probe 1,3,5,7-tetramethyl-8-(3',4'-diaminophenyl)-difluoroboradiaza-s-indacence (DAMBO) is used to label RSNO. The derivatization reaction performs in aqueous medium at 30 degrees C for 15 min in the presence of 1.0 x 10(-4)mol L(-1) Hg(2+). The derivative is detected by fluorescence at an emission wavelength of 507 nm with excitation at 498 nm. The response is a linear function of concentration in the range of 6.0-400.0 x 10(-9)mol L(-1). The detection limit is 1.2 x 10(-10)mol L(-1) (S/N=3). The method is applied to determine RSNO in the blood of healthy persons and patients suffering from cardiovascular diseases. Recoveries of RSNO from spiked blood samples are between 97.52 and 102.81%. The studies indicate that the method presented here is rapid, simple, sensitive and feasible.
亚硝基硫醇（RSNO）已被证明是强效的平滑肌松弛剂和血小板聚集抑制剂。据报道，它是体内一氧化氮（NO）内源性储存和运输的最佳候选物质。生物样品中RSNO的灵敏测定似乎具有重要意义。在这项工作中，提出了一种新颖的荧光光谱法来测定RSNO。使用一种出色的荧光探针1,3,5,7-四甲基-8-（3'，4'-二氨基苯基）-二氟硼二氮杂-s-茚（DAMBO）来标记RSNO。衍生化反应在水介质中，于30℃、在1.0×10⁻⁴mol L⁻¹Hg²⁺存在下进行15分钟。衍生物在激发波长为498nm、发射波长为507nm处通过荧光检测。响应在6.0 - 400.0×10⁻⁹mol L⁻¹范围内是浓度的线性函数。检测限为1.2×10⁻¹⁰mol L⁻¹（S/N = 3）。该方法应用于测定健康人和心血管疾病患者血液中的RSNO。加标血样中RSNO的回收率在97.52%至102.81%之间。研究表明，本文提出的方法快速、简单、灵敏且可行。
